Product Pathways - Metabolism
Hexokinase II (C64G5) Rabbit mAb #2867
PhosphoSitePlus® protein, site, and accession data: HK2
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC | H M R Mk | Endogenous | 102 | Rabbit IgG |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Hexokinase II (C64G5) Rabbit mAb detects endogenous levels of total hexokinase II protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of humanhexokinase II.
Western Blotting
Western blot analysis of extracts from various cell types using Hexokinase II (C64G5) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Hexokinase II (C64G5) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Hexokinase II (C64G5) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
IF-IC
Confocal immunofluorescent analysis of HeLa cells using Hexokinase II (C64G5) Rabbit mAb #2867 (green) and MitoTracker® Red CMXRos, which stains mitochondria, demonstrating colocalization. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
IF-IC
Confocal immunofluorescent analysis of HeLa cells using Hexokinase II (C64G5) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III, and IV (glucokinase), have been identified. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3).
- Warburg, O. (1956) Science 123, 309-314.
- Semenza, G.L. (2000) Crit. Rev. Biochem. Mol. Biol. 35, 71-103.
- Smith, T.A. (2000) Br. J. Biomed. Sci. 57, 170-178.
Application References
- Shackelford, D.B. et al. (2009) Proc Natl Acad Sci U S A 106, 11137-42. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.
