Cell Signaling Technology

Product Pathways - Glucose Metabolism

Hexokinase II (C64G5) Rabbit mAb #2867

Applications Reactivity MW (kDa) Source Isotype
W IHC-P IF-IC H M R Mk 102 Rabbit IgG

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Hexokinase II (C64G5) Rabbit mAb detects endogenous levels of total hexokinase II protein.

Source / Purification

Monoclonal antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of humanhexokinase II.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Hexokinase II (C64G5) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Hexokinase II (C64G5) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Hexokinase II (C64G5) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).


IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Hexokinase II (C64G5) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Background

Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III and IV (glucokinase), have been identified. Hexokinases I, II and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with the rapid cell growth and proliferation (2,3).

  1. Warburg, O. (1956) Science 123, 309-314.
  2. Semenza, G.L. (2000) Crit. Rev. Biochem. Mol. Biol. 35, 71-103.
  3. Smith, T.A. (2000) Br. J. Biomed. Sci. 57, 170-178.

Application References

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