Product Pathways - Tyrosine Kinase/ Adaptors
Phospho-c-Abl (Tyr245) (73E5) Rabbit mAb #2868
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W | H Dm (M) | 135 (c-Abl); 210 (Bcr-Abl) | Rabbit | IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
Dm=D. melanogaster
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-c-Abl (Tyr245) (73E5) Rabbit mAb detects endogenous levels of c-Abl only when phosphorylated at tyrosine 245. The antibody does not cross-react with other activated protein tyrosine kinases.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr245 of human c-Abl.
Western Blotting
Western blot analysis of extracts from cells expressing various activated tyrosine kinase proteins, using Phospho-c-Abl (Tyr245) (73E5) Rabbit mAb (upper) or Phospho-Tyrosine mAb (P-Tyr-100) #9411 (lower).
Western Blotting
Western blot analysis of CML-T1 and BV173 cell lysates, using Phospho-c-Abl (Tyr245) (73E5) Rabbit mAb. The phospho-specificity of the antibody was verified by treating the membrane with (+) (right) or without (-) (left) calf intestinal phosphatase (CIP) after Western transfer.
Background
The c-Abl proto-oncogene encodes a nonreceptor type protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. It is implicated in regulating cell proliferation, differentiation, apoptosis, cell adhesion and stress responses (1-3). c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation, PDGF stimulation and binding to c-Jun, Nck and RFX1 (2,4). The in vivo mechanism of regulation of c-Abl kinase activity is not completely understood. Tyr245 is located in the linker region between the SH2 and catalytic domains. This positioning is conserved among Abl family members. Phosphorylation of Tyr245 is involved in the activation of c-Abl kinase (5). In addition, phosphorylation of Tyr412 which is located in the kinase activation loop of c-Abl is required for kinase activity (6). Thr735 is located within a conserved 14-3-3 protein binding motif region, and can be phosphorylated upon stress stimulation or TPA treatment (Wu, J. et al. unpublished data). Phosphorylation at Thr735 may play an important role in regulating c-Abl localization as well as its function.
- Wang, J.Y. et al. (2000) Oncogene 19, 5643-5650.
- Van Etten, R.A. et al. (1999) Trends Cell. Biol. 9, 179-182.
- Danial, N.N. et al. (2000) Oncogene 19, 2523-2531.
- Shaul, Y. et al. (2000) Cell Death Differ. 7, 10-16.
- Brasher, B.B. et al. (2000) J. Biol. Chem. 275, 35631-35637.
- Pluk, H. et al. (2002) Cell 108, 247-259.
Application References
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Companion Products
- 2861 Phospho-c-Abl (Tyr245) Antibody
- 2862 c-Abl Antibody
- 2864 Phospho-c-Abl (Thr735) Antibody
- 2865 Phospho-c-Abl (Tyr412) (247C7) Rabbit mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
