Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-c-Abl (Tyr245) (73E5) Rabbit mAb #2868

Applications Reactivity Sensitivity MW (kDa) Isotype
W H (M) Endogenous 135 (c-Abl); 210 (Bcr-Abl) Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-c-Abl (Tyr245) (73E5) Rabbit mAb detects endogenous levels of c-Abl only when phosphorylated at tyrosine 245. The antibody does not cross-react with other activated protein tyrosine kinases.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr245 of human c-Abl.

Western Blotting

Western Blotting

Western blot analysis of extracts from cells expressing various activated tyrosine kinase proteins, using Phospho-c-Abl (Tyr245) (73E5) Rabbit mAb (upper) or Phospho-Tyrosine mAb (P-Tyr-100) #9411 (lower).

Western Blotting

Western Blotting

Western blot analysis of CML-T1 and BV173 cell lysates, using Phospho-c-Abl (Tyr245) (73E5) Rabbit mAb. The phospho-specificity of the antibody was verified by treating the membrane with (+) (right) or without (-) (left) calf intestinal phosphatase (CIP) after Western transfer.

Background

The c-Abl proto-oncogene encodes a nonreceptor protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. It is implicated in regulating cell proliferation, differentiation, apoptosis, cell adhesion, and stress responses (1-3). c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation; PDGF stimulation; and binding to c-Jun, Nck, and RFX1 (2,4). The in vivo mechanism for regulation of c-Abl kinase activity is not completely understood. Tyr245 is located in the linker region between the SH2 and catalytic domains. This positioning is conserved among Abl family members. Phosphorylation at Tyr245 is involved in the activation of c-Abl kinase (5). In addition, phosphorylation at Tyr412, which is located in the kinase activation loop of c-Abl, is required for kinase activity (6).

  1. Wang, J.Y. et al. (2000) Oncogene 19, 5643-5650.
  2. Van Etten, R.A. et al. (1999) Trends Cell. Biol. 9, 179-182.
  3. Danial, N.N. et al. (2000) Oncogene 19, 2523-2531.
  4. Shaul, Y. et al. (2000) Cell Death Differ. 7, 10-16.
  5. Brasher, B.B. et al. (2000) J. Biol. Chem. 275, 35631-35637.
  6. Pluk, H. et al. (2002) Cell 108, 247-259.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

For Research Use Only. Not For Use In Diagnostic Procedures.

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