Product Pathways - DNA Damage
ATM (D2E2) XP™ Rabbit mAb #2873
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M | Endogenous | 350 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
Species cross-reactivity is determined by Western blot.
Protocols
Specificity / Sensitivity
ATM (D2E2) XP™ Rabbit mAb detects endogenous levels of total ATM protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human ATM.
Western Blotting
Western blot analysis of extracts of HeLa, NCCIT and PYS2 cells using ATM (D2E2) XP™ Rabbit mAb.
Background
Ataxia telangiectasia mutated kinase (ATM) is a serine/threonine kinase that regulates cell cycle checkpoints and DNA repair (1). Activation of ATM by autophosphorylation on Ser1981 occurs in response to exposed DNA double stranded breaks. ATM kinase regulates a number of proteins involved in cell cycle checkpoint control, apoptosis and DNA repair. Known substrates include p53, Chk2, Chk1, CtIP, 4E-BP1, BRCA1, RPA3, H2AX, SMC1, FANCD2, Rad17, Artemis, Nbs1 and the I-2 regulatory subunit of PP1 (1,2). Mutations in the corresponding ATM gene results in ataxia telangiectasia (AT), an autosomal recessive disease characterized by uncoordinated muscle movement and neurodegeneration. Cells from AT patients display defective DNA damage-induced checkpoint activation, sensitivity to radiation and a higher frequency of chromosome breakage (3,4).
- Lee, J.H. and Paull, T.T. (2007) Oncogene 26, 7741-8.
- Tang, X. et al. (2008) Mol Cell Biol 28, 2559-66.
- Shiloh, Y. (1997) Annu Rev Genet 31, 635-62.
- Petrini, J.H. (2000) Curr Opin Cell Biol 12, 293-6.
Application References
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
