Cell Signaling Technology

Product Pathways - DNA Damage

ATM (D2E2) Rabbit mAb #2873

Applications Reactivity MW (kDa) Source Isotype
W IP H M 350 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

ATM (D2E2) Rabbit mAb detects endogenous levels of total ATM protein.

Source / Purification

Monoclonal antibody is produced by immunizing rabbits with recombinant human ATM.

Western Blotting

Western Blotting

Western blot analysis of extracts of HeLa, NCCIT and PYS2 cells using ATM (D2E2) Rabbit mAb.

Background

Ataxia telangiectasia mutated kinase (ATM) is a serine/threonine kinase that regulates cell cycle checkpoints and DNA repair (1). Activation of ATM by autophosphorylation on Ser1981 occurs in response to exposed DNA double stranded breaks. ATM kinase regulates a number of proteins involved in cell cycle checkpoint control, apoptosis and DNA repair. Known substrates include p53, Chk2, Chk1, CtIP, 4E-BP1, BRCA1, RPA3, H2AX, SMC1, FANCD2, Rad17, Artemis, Nbs1 and the I-2 regulatory subunit of PP1 (1,2). Mutations in the corresponding ATM gene results in ataxia telangiectasia (AT), an autosomal recessive disease characterized by uncoordinated muscle movement and neurodegeneration. Cells from AT patients display defective DNA damage-induced checkpoint activation, sensitivity to radiation and a higher frequency of chromosome breakage (3,4).

  1. Lee, J.H. and Paull, T.T. (2007) Oncogene 26, 7741-8.
  2. Tang, X. et al. (2008) Mol Cell Biol Epub ahead of print.
  3. Shiloh, Y. (1997) Annu Rev Genet 31, 635-62.
  4. Petrini, J.H. (2000) Curr Opin Cell Biol 12, 293-6.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

Product Pathways

Drug Discovery Tools

Featured Technologies

Protein Classes