Cell Signaling Technology

Product Pathways - Neuroscience

CASK Antibody #2878

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 105 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

CASK Antibody detects endogenous levels of total CASK protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminus of CASK. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brain, using CASK Antibody.

Background

CASK is an adaptor protein with a calcium/calmodulin-dependent protein kinase domain, a SH3 domain, a guanylate kinase homology domain (GUK) and a PDZ domain. CASK links transmembrane proteins to the cytoskeleton and signaling molecules. In particular, CASK binds to Neurexin to stabilize pre- and post-synaptic structures (1). While most of CASK proteins (~80%) are cytoplasmic, a portion of the protein enters the nucleus, where it acts as a transcriptional coactivator (2). Manifesting the importance of CASK, transgenic mice with insertional mutations die within 24 hours of birth (3).

  1. Hata, Y. et al. (1996) J Neurosci 16, 2488-94.
  2. Hsueh, Y.P. et al. (2000) Nature 404, 298-302.
  3. Laverty, H.G. and Wilson, J.B. (1998) Genomics 53, 29-41.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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