Cell Signaling Technology

Product Pathways - Translational Control

ERp57 (G117) Antibody #2881

Applications Reactivity MW (kDa) Source
W H M R 57 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

ERp57 (G117) Antibody detects endogenous levels of total ERp57 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence around Gly117 of human ERp57. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using ERp57 (G117) Antibody.

Background

Secretory proteins translocate into the endoplasmic reticulum (ER) after their synthesis where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Disulfide isomerase (PDI) has two thioredoxin homology domains and catalyzes the formation and isomerization of these disulfide bonds (2). Other ER resident proteins that possess the thioredoxin homology domains, including endoplasmic reticulum stress protein 57 (ERp57), constitute the PDI family (2). ERp57 interacts with calnexin and calreticulin (3) and is suggested to play a role in the isomerization of disulfide bonds on certain glycoproteins (3).

  1. Huppa, J.B. and Ploegh, H.L. (1998) Cell 92, 145-8.
  2. Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep 6, 28-32.
  3. Jessop, C.E. et al. (2007) EMBO J 26, 28-40.

Application References

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