Cell Signaling Technology

Product Pathways - Wnt / Hedgehog / Notch

TCF3 (D15G11) XP™ Rabbit mAb #2883

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Mk Endogenous 78 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

TCF3 (D15G11) XP™ Rabbit mAb detects endogenous levels of total TCF3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln96 of human TCF3.

Western Blotting

Western Blotting

Western blot analysis of total lysates from various cell lines using TCF3 (D15G11) XP™ Rabbit mAb.

Background

LEF1 and TCF are members of the high mobility group (HMG) DNA binding protein family of transcription factors which consists of the following: Lymphoid enhancer factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3 and TCF4 (1). LEF1 and TCF1 were originally identified as important factors regulating early lymphoid development (2) that act downstream in Wnt signaling. LEF1 and TCF bind to Wnt response elements to provide docking sites for β-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling (3). LEF1 and TCF proteins are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers including colon cancer (4,5).

TCF3 (also known as TCF7L1) plays an important role in integrating Wnt signaling with control of stem cell differentiation (6-8).

  1. Waterman, M.L. (2004) Cancer Metastasis Rev. 23, 41-52.
  2. Schilham, M.W. and Clevers, H. (1998) Semin. Immunol. 10, 127-132.
  3. Brantjes, H. et al. (2002) Biol. Chem. 383, 255-261.
  4. Reya, T. and Clevers, H. (2005) Nature 434, 843-850.
  5. Logan, C.Y. and Nusse, R. (2004) Annu. Rev. Cell Dev. Biol. 20, 781-810.
  6. Merrill, B.J. et al. (2001) Genes Dev. 15, 1688-1705.
  7. Nguyen, H. et al. (2006) Cell 127, 171-183.
  8. Cole, M.F. et al. (2008) Genes Dev. 22, 746-755.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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