Cell Signaling Technology

Product Pathways - NF-kB Signaling

ERC1α (D1055) Antibody #2885

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Endogenous 130 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ERC1α (D1055) Antibody detects endogenous levels of the α isoform of human ERC1.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxy terminus of human ERC1 protein, specific for the α isoform. Antibodies were purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from ACHN, HeLa and SK-MEL-5 cells using ERC1α (D1055) Antibody.

Background

ERC1, an acronym named for previous protein names ELKS (1), RAB6IP2 (2) and CAST (3), is a RIM-binding protein that plays a role in neurotransmitter release and general membrane trafficking in other cell types (2-5). Interaction with the GTP-binding protein Rab6 suggests that it contributes to membrane traffic at the Golgi (2). In addition to its association with membrane trafficking, ERC1 has also been found as an essential part of the IκB kinase (IKK) complex required for the activation of NF-κB, perhaps by recruiting IκBα to the IKK complex (6). Alternative splicing of ERC1 generates 2 proteins with a divergent carboxy terminus, a long and a short form termed ERC1α and ERC1β, respectively. ERC1α is widely expressed, whereas ERC1β and a related family member ERC2 are expressed in the brain (4). Papillary thyroid carcinomas have been identified with the translocation t(10;12)(p11;p13) resulting in a fusion between ERC1 and the receptor tyrosine kinase Ret (1).

  1. Nakata, T. et al. (1999) Genes Chromosomes Cancer 25, 97-103.
  2. Monier, S. et al. (2002) Traffic 3, 289-97.
  3. Ohtsuka, T. et al. (2002) J Cell Biol 158, 577-90.
  4. Wang, Y. et al. (2002) Proc Natl Acad Sci USA 99, 14464-9.
  5. Ohara-Imaizumi, M. et al. (2005) Mol Biol Cell 16, 3289-300.
  6. Ducut Sigala, J.L. et al. (2004) Science 304, 1963-7.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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