Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Calreticulin Antibody #2891

Applications Reactivity Sensitivity MW (kDa) Source
W IF-IC H M R Mk Endogenous 55 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

Calreticulin Antibody detects endogenous levels of total calreticulin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Gln65 of human calreticulin. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and C6 cells using Calreticulin Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Calreticulin Antibody (green). Actin filaments have been labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ™ (fluorescent DNA dye).

Background

Calcium is a universal signaling molecule involved in many cellular functions such as cell motility, metabolism, protein modification, protein folding and apoptosis. Calcium is stored in the endoplasmic reticulum (ER), where it is buffered by calcium binding chaperones such as calnexin and calreticulin, and is released via the IP3 Receptor (1). Calreticulin also functions as an ER chaperone that ensures proper folding and quality control of newly synthesized glycoproteins. As such, calreticulin presumably does not alter protein folding but ensures proper timing for efficient folding and subunit assembly. Furthermore, calreticulin retains proteins in non-native conformation within the ER and targets them for degradation (2,3).

  1. Groenendyk, J. et al. (2004) Mol Cells 17, 383-9.
  2. Nauseef, W.M. et al. (1995) J Biol Chem 270, 4741-7.
  3. Williams, D.B. (2006) J Cell Sci 119, 615-23.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Products