Product Pathways - Cell Cycle / Checkpoint
p18 INK4C (DCS118) Mouse mAb #2896
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IP | H | 18 | Mouse | IgG2a |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
The p18 INK4C (DCS118) Mouse mAb detects endogenous levels of p18 INK4C protein. The antibody does not cross-react with other proteins of the INK4 family.
Source / Purification
Monoclonal antibody is produced by immunizing mice with purified recombinant human p18 INK4C protein.
Western Blotting
Western blot analysis of extracts from HeLa, 293 and Ramos cells, using p18 INK4C (DCS118) Mouse mAb.
Background
Cyclin-dependent kinases (CDKs) are activated in part by forming complexes with cyclins. For example, CDK4 and CDK6 associate with the D-type cyclins and phosphorylate the retinoblastoma protein. This phosphorylation is a necessary event for cells to enter S-phase (1). The inhibitors of CDK4 (INK4) family include p15 INK4B, p16 INK4A, p18 INK4C and p19 INK4D. p18 has been shown to function as a haploinsufficient tumor suppressor in vivo (2). All INK4 proteins are composed of 32 amino acid ankyrin motifs and selectively inhibit CDK4/6 activity. Mutational analyses of p18 implicate the third and the amino-terminal portion of the fourth ankyrin repeat in mediating binding to CDK4/6 (3). The interaction of INK4 family members can be a binary complex with CDK4/6 or ternary complex with cyclin D-bound CDK4/6 and ultimately results in the inhibition of cell cycle progression (4,5).
- Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-6925.
- Bai, F. et al. (2003) Mol. Cell. Biol. 23, 1269-1277.
- Noh, S.J. et al. (1999) Cancer Res. 59, 558-564.
- Guan, K.L. et al. (1994) Genes Dev. 8, 2939-2952.
- Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681.
Application References
- Bartkova, J. et al. (2000) Cell cycle regulators in testicular cancer: loss of p18 INK4C marks progression from carcinoma in situ to invasive germ cell tumours. Int. J. Cancer 85 (3), 370-375. This article references the use of p18 INK4C (DCS118) Mouse mAb in the following applications: IHC-P (paraffin) Western Blotting
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