Cell Signaling Technology

Product Pathways - Tyrosine Kinase/ Adaptors

VEGF Receptor 2 Control Proteins #2904

Molecular Weight

110 kDa recombinant kinase fragment

Description

Nonphosporylated VEGFR2 Cell Control Proteins: Purified recombinant human VEGFR2 (Val789-Val1356) expressed as a GST fusion protein in insect cells treated with λ phosphatase. Supplied in SDS Sample Buffer. Store at -20°C.Phosphorylated VEGFR2 Cell Extracts: Purified recombinant human VEGFR2 (Val789-Val1356) expressed as a GST fusion protein in insect cells. Supplied in SDS Sample Buffer. Store at -20°C.

Directions for Use

As controls, we recommend using 15 µl (20 ng) of phosphorylated and nonphosphorylated VEGFR2 control proteins. Boil sample before use.

Western Blotting

Western Blotting

Western blot analysis of recombinant human GST-VEGF Receptor 2 (Val789-Val1356), untreated or λ phosphatase-treated, using Phospho-VEGF Receptor 2 (Tyr1175) Antibody #2478 (upper), VEGF Receptor 2 Antibody # 2479 (lower).

Background

Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The phosphorylation of Tyr1212 provides a docking site for Grb2 binding and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).

  1. Meyer, M. et al. (1999) EMBO J. 18, 363-374.
  2. Dougher-Vermazen, M. et al. (1994) Biochem. Biophys. Res. Commun. 205, 728-738.
  3. Kroll, J. and Waltenberger, J. (1997) J. Biol. Chem. 272, 32521-32527.
  4. Takahashi, T. et al. (2001) EMBO J. 20, 2768-2778.
  5. Holmqvist, K. et al. (2004) J. Biol. Chem. 279, 22267-22275.
  6. Karkkainen, M.J. and Petrova, T. (2000) Oncogene 19, 5598-5605.
  7. Rahimi, N. et al. (2000) J. Biol. Chem. 275, 16986-16992.
  8. Claesson-Welsh, L. (2003) Biochem. Soc. Trans. 31, 20-24.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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