Product Pathways - Cell Cycle / Checkpoint
CDK4 (DCS156) Mouse mAb #2906
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W | H M | 30 | Mouse | IgG1 |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
CDK4 (DCS156) Mouse mAb detects endogenous levels of total CDK4 protein. The antibody does not cross-react with other CDKs.
Source / Purification
Monoclonal antibody is produced by immunizing mice with recombinant human CDK4 corresponding to amino acids 270-290.
Western Blotting
Western blot analysis of extracts from SK-N-MC and IMCD3 cells, using CDK4 (DCS156) Mouse mAb.
Background
Cyclin-dependent kinase activity is regulated by T-loop phosphorylation, by the abundance of their cyclin partners and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase (2). The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). In HeLa cells, upon UV irradiation, upregulation of p16 INK4a association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclinD3 activity in progression through G2-phase of the cell cycle (4).
- Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681.
- Sherr, C.J. (1996) Science 274, 1672-1677.
- Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-6925.
- Gabrielli, B.G. et al. (1999) J. Biol. Chem. 274, 13961-13969.
Application References
- Lukas, C. et al. (1999) Immunochemical analysis of the D-type cyclin-dependent kinases cdk4 and cdk6, using a series of monoclonal antibodies. Hybridoma 18 (3), 225-234. This article references the use of CDK4 (DCS156) Mouse mAb in the following applications: IC-IF IHC-P (paraffin) Western Blotting
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