Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-Catenin δ-1 (Tyr904) Antibody #2910

Applications Reactivity MW (kDa) Source
W H 95, 100 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-Catenin δ-1 (Tyr904) Antibody detects endogenous levels of catenin δ-1 protein only when phosphorylated at Tyr904. The antibody might cross react with another overexpressed phospho-tyrosine protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with synthetic phosphopeptides (KLH-coupled) corresponding to residues surrounding Tyr904 of human/mouse catenin δ-1. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from overnight serum starved A431 cells, untreated or EGF-treated for 15 minutes, using Phospho-Catenin δ-1 (Tyr904) Antibody (upper) and Catenin δ-1 Antibody #4989 as a loading control (lower).

Background

Catenin δ-1 (p120 catenin) has an amino-terminal coiled-coil domain followed by a regulatory domain containing multiple phosphorylation sites and a central Armadillo repeat domain of ten, linked 42 amino-acid repeats. The carboxy-terminal tail has no known function (1). Catenin δ-1 fulfills critical roles in the regulation of cell-cell adhesion as it regulates E-cadherin turnover at the cell surface to determine the level of E-cadherin available for cell-cell adhesion (2). Catenin δ-1 has both positive and negative effects on cadherin-mediated adhesion (3). Actin dynamics are also regulated by catenin δ-1, which modulates RhoA, Rac and cdc42 proteins (1). Analogous to β-catenin, catenin δ-1 translocates to the nucleus although its role at this location is unclear. Many studies show that catenin δ-1 is expressed irregularly or is absent in various types of tumor cells, suggesting that catenin δ-1 may function as a tumor suppressor (4).

Phosphorylation of Tyr904 on Catenin-δ-1 was identified at Cell Signaling Technology (CST) using PhosphoScan®, a CST's LC-MS/MS platform for phosphorylation site discovery (5).

  1. Reynolds, A.B. and Roczniak-Ferguson, A. (2004) Oncogene 23, 7947-7956.
  2. Davis, M. A. et al. (2003) J. Cell Biol. 163, 525-534.
  3. Thoreson, M.A. and Reynolds, A.B. (2002) Differentiation 70, 583-589.
  4. Anastasiadis, P.Z. and Reynolds, A.B. (2000) J. Cell Sci. 113, 1319-1334.
  5. Rush, J. et al. (2005) Nat. Biotechnol. 23, 94-101.

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