Product Pathways - Cell Cycle / Checkpoint
CDK7 (MO1) Mouse mAb #2916
PhosphoSitePlus® protein, site, and accession data: CDK7
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P | H R | Endogenous | 40 | Mouse IgG2b |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
CDK7 (MO1) Mouse mAb detects endogenous levels of total CDK7 protein. The antibody does not cross-react with other CDKs.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human CDK7.
Western Blotting
Western blot analysis of extracts from SK-N-MC and C6 cells, using CDK7 (MO1) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using CDK7 (MO1) Mouse mAb.
Background
CDK-activating kinase (CAK) is a complex of CDK7 and cyclin H. The complex is involved in cell cycle regulation by phosphorylating an activating residue in the T-loop domain of cdks (1). Regulation of CAK activity is mediated by T-loop phosphorylation and by association with MAT1, both of which enhance its kinase activity toward the CTD of RNA polymerase II (2,3) and other substrates such as p53 (4). CAK is an essential component of the transcription complex TFIIH and may interact directly with TFIIH helicases (5).
- Fisher, R.P. et al. (1994) Cell 78, 713-724.
- Larochelle, S. et al. (2001) EMBO J. 16, 3749-3759.
- Yankulov, K.Y. et al. (1997) EMBO J. 16, 1638-1646.
- Ko, L.J. et al. (1997) Mol. Cell. Biol. 17, 7220-7229.
- Rossignol, M. et al. (1997) EMBO J. 16, 1628-1637.
Application References
- Bartkova, J. et al. (1996) Expression of cdk7/CAK in normal and tumour cells of diverse histogenesis, cell-cycle position and differentiation. Int. J. Cancer 66, 732-737. Applications: IC-IF Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.
