Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Cyclin D1 Antibody #2922

Applications Reactivity MW (kDa) Source
W IP H M R 36 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Cyclin D1 Antibody detects endogenous levels of cyclin D1. It does not cross-react with other family members.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues near the carboxy terminus of cyclin D1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from C6 and L929 cells, using Cyclin D1 Antibody.

Background

Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to effect progression through the restriction point and pRb-dependent entry into S-phase (2). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of its protein expression and through phosphorylation-dependent degradation (4).

  1. Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681.
  2. Sherr, C.J. (1996) Science 274, 1672-1677.
  3. Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-6925.
  4. Diehl, J.A. et al. (1997) Genes Dev. 11, 957-972.

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