Product Pathways - Cell Cycle / Checkpoint
Cyclin D1 (DCS6) Mouse mAb #2926
|W IP||H M R||Endogenous||36||Mouse IgG2a|
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Cyclin D1 (DCS6) Mouse mAb detects endogenous levels of total cyclin D1 protein. The antibody does not cross-react with cyclin D2 or D3.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human cyclin D1 corresponding to residues 151-170.
Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase (2). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation (4).
Of the three D cyclins, aberrant expression of cyclin D1 has been associated with many forms of cancer including B cell lymphomas and can directly contribute to oncogenesis by various mechanisms including gene translocation or amplification (2). Cyclin D1 also plays a critical role in mammary tissue maturation (5).
- Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681.
- Sherr, C.J. (1996) Science 274, 1672-1677.
- Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-6925.
- Diehl, J.A. et al. (1997) Genes Dev. 11, 957-972.
- Sicinski, P. et al. (1995) Cell 82, 621-630.
- Lukas, J. et al. (1994) Cyclin D1 protein oscillates and is essential for cell cycle progression in human tumour cell lines. Oncogene 9, 707-718. Applications: IC-IF Western Blotting
- Bartkova, J. et al. (1995) Abnormal patterns of D-type cyclin expression and G1 regulation in human head and neck cancer. Cancer Res. 55, 949-956. Applications: IHC-P (paraffin)
- Chang, S.F. et al. (2008) Proc Natl Acad Sci U S A 105, 3927-32. Applications: Western Blotting
- Tian, J. et al. (2012) J Clin Invest 122, 192-204. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.