Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Cyclin H Antibody #2927

Applications Reactivity Sensitivity MW (kDa) Source
W IP IHC-P H M R Endogenous 36 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Cyclin H Antibody detects endogenous levels of cyclin H. It does not cross-react with other family members at physiological levels.

Source / Purification

Polyclonal antibodies are prepared by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues near the carboxy-terminus of cyclin H. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat and Raji cells, using Cyclin H Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis pf paraffin-embedded human colon carcinoma, showing nuclear localization, using Cyclin H Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Jurkat cells, using Cyclin H Antibody.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis pf paraffin-embedded human lung carcinoma, using Cyclin H Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis pf paraffin-embedded human colon carcinoma, using Cyclin H Antibody in the presence of control peptide (left) or antigen specific peptide (right),

Background

Cyclin H belongs to a conserved cyclin family that plays a critical role in the regulation of cell cycle dependent kinases (CDKs) necessary for cell cycle progression (1,2). In general, the activity of CDKs requires the binding of appropriate cyclins as well as phosphorylation driven by Cdk-activating kinase (CAK). Cyclin H is part of the CAK complex that includes the kinase CDK7, and an assembly factor p36/Mat1, which enhances binding between cyclin H and CDK7 and increases activity (3,4). CAK regulates progression through the cell cycle by activating cdc2, CDK2, and CDK4 kinases through phosphorylation of a critical threonine residue in the T-loop of the CDK-cyclin complexes (5,6). The CAK complex can exist either in its free form or in association with transcription factor IIH (TFIIH) which can affect its substrate specificity (7,8,9). When bound to TFIIH, CAK preferentially phosphorylates the carboxy-terminal domain of RNA polymerase II (9), providing a link between cell cycle control, transcriptional regulation, and DNA repair.

  1. Fisher, R.P. and Morgan, D.O. (1994) Cell 78, 713-724.
  2. Makela, T. P. et al. (1994) Nature 371, 254-257.
  3. Yee, A. et al. (1995) Cancer Res. 55, 6058-6062.
  4. Devault, A. et al. (1995) EMBO J. 14, 5027-5036.
  5. Solomon, M.J. (1994) Trends Biochem. Sci. 19, 496-500.
  6. Morgan, M.O. (1995) Nature 374, 131-133.
  7. Shiekhatter, R. et al. (1995) Nature 374, 283-287.
  8. Serizawa, H. et al. (1995) Nature 374, 280-282.
  9. Rossignol, M. et al. (1997) EMBO J. 16, 1628-1637.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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