Cell Signaling Technology

Product Pathways - Apoptosis

DAPK3/ZIPK Antibody #2928

Applications Reactivity Sensitivity MW (kDa) Source
W H M R (Mk) Endogenous 52 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

DAPK3/ZIPK Antibody detects endogenous levels of total DAPK3/ZIPK protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxyl terminus of human DAPK3/ZIPK. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using DAPK3/ZIPK Antibody.

Background

Death-associated protein kinase (DAPK1) is a Ca2+/calmodulin-regulated serine/threonine kinase that participates in a wide range of apoptotic signals including interferon-γ, tumor necrosis factor α, Fas, activated c-Myc, and detachment from the extracellular matrix. In addition to the kinase domain and calmodulin regulatory segment, DAPK1 also has eight ankyrin repeats, a cytoskeleton binding region, and a conserved death domain (1-3). Deletion of the calmodulin-regulatory domain generates a constitutively active mutant kinase. Ectopic expression of wild-type DAPK1 induced cell death in HeLa cells. Conversely, expression of a catalytically inactive mutant protected cells from interferon-γ-induced cell death (4). The catalytic domain of DAPK1 has very high sequence similarity to vertebrate myosin light chain kinase (MLCK) and a RXX(S/T)X motif derived from myosin light chain protein was shown to be phosphorylated in vitro by DAPK1 (5).

The DAPK family consists of several kinases including DAPK, DAPK2/DRP-1 (6), and DAPK3/ZIPK/DLK (7-9) with homology in their catalytic domain. Overexpression of DAPK3/ZIPK, but not a catalytically inactive mutant, can induce apoptosis (7). DAPK3 was also identified as a myosin light chain kinase, demonstrating ability to phosphorylate the regulatory light chain of myosin II in a Ca2+/calmodulin-independent manner (8). In addition to an amino-terminal kinase domain, DAPK3 contains a carboxy-terminal leucine zipper domain that mediates interaction with leucine zipper transcription factors such as ATF4 (7). DAPK3 is predominantly localized to the nucleus and has been found in PML oncogenic domains (PODs) associated with DAXX and PAR-4, and can phosphorylate PAR-4 in vitro (10,11). In addition, DAPK3 can phosphorylate STAT3 at Ser727 to enhance its transcriptional activity (12).

  1. Kimchi, A. (1999) Ann Rheum Dis. 58, I14-I19.
  2. Cohen, O. et al. (1999) J Cell Biol 146, 141-148.
  3. Deiss, L. P. et al. (1995) Genes Dev 9, 15-30.
  4. Cohen, O. et al. (1997) EMBO J 16, 998-1008.
  5. Velentza, A. V. et al. (2001) J Biol Chem 276, 38956-38965.
  6. Inbal, B. et al. (2000) Mol Cell Biol 20, 1044-54.
  7. Kawai, T. et al. (1998) Mol Cell Biol 18, 1642-51.
  8. Murata-Hori, M. et al. (1999) FEBS Lett 451, 81-4.
  9. Kögel, D. et al. (1998) Oncogene 17, 2645-54.
  10. Page, G. et al. (1999) Oncogene 18, 7265-73.
  11. Kawai, T. et al. (2003) Mol Cell Biol 23, 6174-86.
  12. Sato, N. et al. (2005) Int Immunol 17, 1543-52.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products