Product Pathways - Adhesion
EpCAM (VU1D9) Mouse mAb #2929
PhosphoSitePlus® protein, site, and accession data: EPCAM
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC F | H | Endogenous | 40 | Mouse IgG1 |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
EpCAM (VU1D9) Mouse mAb detects endogenous levels of total EpCAM protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with NCI-H69 small cell lung carcinoma cells.
Western Blotting
Western blot analysis of extracts from HT-29 and HCT-15 cells using EpCAM (VU1D9) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using EpCAM (VU1D9) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon using EpCAM (VU1D9) Mouse mAb.
Flow Cytometry
Flow cytometric analysis of unpermeabilized HT-29 cells using EpCAM (VU1D9) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HT-29 cells using EpCAM (VU1D9) Mouse mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca2+-independent, homophilic adhesions on the basolateral surface of most epithelial cells. EpCAM is not expressed in adult squamous epithelium, but it is highly expressed in adeno and squamous cell carcinomas (1). One of the first tumor-associated antigens discovered, EpCAM has long been a marker of epithelial and tumor tissue. Because of its high expression in cancer, EpCAM has become a therapeutic target for human solid tumors of epithelial lineage (reviewed in 2,3).
- Went, P.T. et al. (2004) Hum Pathol 35, 122-8.
- Baeuerle, P.A. and Gires, O. (2007) Br J Cancer 96, 417-23.
- Armstrong, A. and Eck, S.L. Cancer Biol Ther 2, 320-6.
Application References
- Luk, J.M. et al. (2011) J Biol Chem 286, 30706-13. Applications: IHC-P (paraffin)
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
