Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-PSD93 (Tyr340) Antibody #2930

Applications Reactivity Sensitivity MW (kDa) Source
W IP R Endogenous 110 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-PSD93 (Tyr340) Antibody detects endogenous levels of PSD93 only when phosphorylated at Tyr340.

Source / Purification

Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr340 of human PSD93.

Western Blotting

Western Blotting

Western blot analysis of extracts from rat brain, either sham-operated, 15 min ischemia followed by 4 h reperfusion, or 15 min ischemia only, using Phospho-PSD93 (Tyr340) Antibody (upper) or PSD95 Antibody (lower).

Background

Postsynaptic Density Protein 93 (PSD93) is a member of the PSD subfamily of the membrane-associated guanylate kinase (PSD-MAGUK) proteins. Structurally, it most closely resembles PSD95, consisting of an N-terminal variable segment followed by three PDZ domains, an SH3 domain, and an inactive guanylate kinase (GK) domain (1,2). PSD93 is expressed in neuronal cells and located at the synapse where it interacts with neuronal receptors and proteins including the NMDA receptor (2-4), K+ channels (5,6), and the AMPA receptor (7) to regulate their membrane localization and neuronal signaling. Research studies have implicated PSD93 in postsynaptic related persistent pain induction, making PSD93 a potential target for treatment of this syndrome (3).

The phosphorylation site at Tyr340 of PSD93 was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's MS/MS platform for phosphorylation site discovery. Phosphorylation of PSD93 at Tyr340 was observed in extracts isolated from reperfusion of ischemic rat brain.

  1. Brenman, J.E. et al. (1996) J Neurosci 16, 7407-15.
  2. Kim, E. et al. (1996) Neuron 17, 103-13.
  3. Tao, Y.X. et al. (2003) J Neurosci 23, 6703-12.
  4. Niethammer, M. et al. (1996) J Neurosci 16, 2157-63.
  5. Kim, E. et al. (1995) Nature 378, 85-8.
  6. Leyland, M.L. and Dart, C. (2004) J Biol Chem 279, 43427-36.
  7. Elias, G.M. et al. (2006) Neuron 52, 307-20.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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