Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Cyclin D3 (DCS22) Mouse mAb #2936

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P H M R Endogenous 31 Mouse IgG1

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Cyclin D3 (DCS22) Mouse mAb detects endogenous levels of total cyclin D3 protein. The antibody does not cross-react with cyclin D1 or cyclin D2.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human cyclin D3 corresponding to residues 241-260.

Western Blotting

Western Blotting

Western blot analysis of extracts from SK-N-MC, C6 and IMCD3 cells, using Cyclin D3 (DCS22) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Cyclin D3 (DCS22) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Cyclin D3 (DCS22) Mouse mAb.


Background

Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase (2). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation (4).

Although the D-type cyclins are not fully redundant, cyclin D3, like D1, plays a prominent role in differentiation and proliferation, which correlates with higher expression levels of cyclin D3 in various cancers (5).

  1. Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681.
  2. Sherr, C.J. (1996) Science 274, 1672-1677.
  3. Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-6925.
  4. Diehl, J.A. et al. (1997) Genes Dev. 11, 957-972.
  5. Bartkova, J. et al. (1998) Oncogene 17, 1027-37.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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