Product Pathways - Cell Cycle / Checkpoint
Cyclin D3 (DCS22) Mouse mAb #2936
|2936S||100 µl (20 western blots)||---||In Stock||---|
|2936P||40 µl (8 western blots)||---||In Stock||---|
|2936||carrier free and custom formulation / quantity||email request|
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|W||1:2000||Human, Mouse, Rat||Endogenous||31||Mouse IgG1|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin)
Specificity / Sensitivity
Cyclin D3 (DCS22) Mouse mAb detects endogenous levels of total cyclin D3 protein. The antibody does not cross-react with cyclin D1 or cyclin D2.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human cyclin D3 corresponding to residues 241-260.
Western blot analysis of extracts from SK-N-MC, C6 and IMCD3 cells, using Cyclin D3 (DCS22) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Cyclin D3 (DCS22) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Cyclin D3 (DCS22) Mouse mAb.
Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase (2). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation (4).
Although the D-type cyclins are not fully redundant, cyclin D3, like D1, plays a prominent role in differentiation and proliferation, which correlates with higher expression levels of cyclin D3 in various cancers (5).
- Bartkova, J. et al. (1998) Oncogene 17, 1027-37. Applications: Western Blotting, IC-IF.
- Bartkova, J. et al. (2001) Gastroenterology 120, 1680-1688. Applications: IHC-P (paraffin).
- Bartkova, J. et al. (1996) Int. J. Cancer 65, 323-327. Applications: Western Blotting, IC-IF, IHC-P (paraffin).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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