Cell Signaling Technology

Product Pathways - Apoptosis

TP/ECGF1 Antibody #2937

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 50, 60 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TP/ECGF1 Antibody detects endogenous levels of human TP/ECGF1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residues surrounding Gln370 of human TP/ECGF1. Antibodies are purified by peptide affinity chromatography.

Western blot analysis of extracts from COS cells transfected with human ECGF1 using TP/ECGF1 Antibody.

Western blot analysis of extracts from human THP-1 cells, untreated or LPS-treated overnight, using TP/ECGF1 Antibody.

Background

Thymidine phosphorylase (TP) is a platelet-derived endothelial cell growth factor (PD-ECGF) that catalyzes the formation of thymine and 2-deoxy-D-ribose-1-phosphate from thymidine and orthophosphate (1). This intracellular enzyme is capable of both promoting angiogenesis and inhibiting apoptosis. Thymidine phosphorylase catalytic activity is required for its angiogenic function (2,3). Increased expression of TP/PD-ECGF is seen in a wide variety of different solid tumors and inflammatory diseases and is often associated with poor prognosis (4,5). Alternatively, TP can activate fluorouracil derivative (DFUR) prodrugs and increase the antitumor activity of the related treatment (1,5). The use of thymidine phosphorylase as a cancer therapeutic target has been studied extensively, with emphasis on either inhibiting TP enzymatic activity or increasing enzyme induction with concomitant DFUR treatment (1,5).

  1. Rooseboom, M. et al. (2004) Pharmacol Rev 56, 53-102.
  2. Moghaddam, A. and Bicknell, R. (1992) Biochemistry 31, 12141-6.
  3. Furukawa, T. et al. (1992) Nature 356, 668.
  4. Toi, M. et al. (2005) Lancet Oncol 6, 158-66.
  5. Liekens, S. et al. (2007) Biochem Pharmacol 74, 1555-67.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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