Cell Signaling Technology

Product Pathways - Translational Control

p58IPK (C56E7) Rabbit mAb #2940

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M Mk Endogenous 58 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

p58IPK (C56E7) Rabbit mAb detects endogenous levels of total p58IPK protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human p58IPK.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using p58IPK (C56E7) Rabbit mAb.

Background

p58IPK is an inhibitor of interferon-induced and double-stranded RNA-activated protein kinase (PKR). It physically interacts with PKR and inhibits its activation and activity (1). Influenza virus activates p58IPK and thus blocks the activity of PKR to repress translation in the infected cells. In the uninfected cells, p58IPK forms a complex with its own inhibitor, HSP40, and is kept in an inactive state (2). ER stress induces the expression of p58IPK mediated by ATF6 (3,4). The induced p58IPK negatively regulates Perk activity, inhibits eIF2α phosphorylation and suppresses the activation of expression of downstream ER-responsive genes ATF4 and GADD153 (4). More recently, p58IPK has been shown to associate with the lumen of the endoplasmic reticulum (ER) where it is believed to serve as a cochaperone for BiP (5).

  1. Gale, M. et al. (1996) Mol Cell Biol 16, 4172-81.
  2. Melville, M.W. et al. (1997) Proc Natl Acad Sci USA 94, 97-102.
  3. Yan, W. et al. (2002) Proc Natl Acad Sci USA 99, 15920-5.
  4. van Huizen, R. et al. (2003) J Biol Chem 278, 15558-64.
  5. Rutkowski, D.T. et al. (2007) Mol Biol Cell 18, 3681-91.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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