Cell Signaling Technology

Product Pathways - Development

β-Catenin Antibody Sampler Kit #2951

Kit Includes Quantity Applications Reactivity MW (kDa) Isotype
β-Catenin (D10A8) XP® Rabbit mAb #8480 40 µl W IP IHC-P IHC-F IF-F IF-IC F ChIP H M R Mk (Z) (B) (Pg) (GP) (Hr) 92 Rabbit IgG
Phospho-β-Catenin (Ser33/37/Thr41) Antibody #9561 40 µl W H M R Mk (C) (X) (Z) (B) (Dg) (Pg) 92 Rabbit
Phospho-β-Catenin (Thr41/Ser45) Antibody #9565 40 µl W H M Mk (Dg) (Pg) 92 Rabbit
Phospho-β-Catenin (Ser552) (D8E11) Rabbit mAb #5651 40 µl W IP H (M) (R) (X) (Z) 92 Rabbit IgG
Phospho-β-Catenin (Ser675) (D2F1) XP® Rabbit mAb #4176 40 µl W IP IF-F IF-IC H (M) (R) (C) (X) (Z) 92 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl Goat

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)  IF-F=Immunofluorescence (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry  ChIP=Chromatin IP
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  C=Chicken  X=Xenopus  Z=Zebrafish  B=Bovine  Dg=Dog  Pg=Pig  GP=Guinea Pig  Hr=Horse
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

Each antibody in this kit recognizes only its specific target and does not cross-react with other family members.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, pretreated with 20 mM LiCl for 30 minutes and then with 50 nM calyculin A, using Phospho-β-Catenin (Ser33/37/Thr41) Antibody #9561.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells pretreated with 20 mM LiCl for 30 minutes and then with 50 nM calyculin A, using Phospho-β-Catenin (Thr41/Ser45) Antibody #9565.

Western Blotting

Western Blotting

Western blot analysis of extracts from MKN-45 and SK-N-MC cells, untreated or treated with λ phosphatase for 1 hour or forskolin (FSK) for 30 minutes, using Phospho-β-Catenin (Ser675) (D2F1) XP® Rabbit mAb #4176(upper) or β-Catenin (6B3) Rabbit mAb #9582 (lower).


Western Blotting

Western Blotting

Western blot analysis of extracts from SK-N-MC and NTERA-2 cells, untreated or λ phosphatase-treated, using Phospho-β-Catenin (Ser552) (D8E11) Rabbit mAb #5651 (upper) or β-Catenin (6B3) Rabbit mAb #9582 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using β-Catenin (D10A8) XP® Rabbit mAb #8480.

Description

The β-Catenin Antibody Sampler Kit provides an economical means of detecting total β-catenin as well as β-catenin phosphorlylated at various residues. The kit contains enough primary and secondary antibody to perform four Western blots with each antibody.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser33, Ser37 and Thr41 of human β-catenin or residues surrounding Thr41 and Ser45 of human β-catenin. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro714 of human β-catenin protein, a synthetic phosphopeptide corresponding to residues surrounding Ser552 of human β-catenin protein, or a synthetic phosphopeptide corresponding to residues surrounding Ser675 of human β-catenin.

Background

β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

Both Akt and PKA were shown to phosphorylate β-catenin at Ser552 and Ser675. Phosphorylation at Ser552 and Ser675 induces β-catenin accumulation in the nucleus and increases its transcriptional activity (9-12).

  1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305.
  2. Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell. Dev. Biol. 14, 59-88.
  3. Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.
  4. Amit, S. et al. (2002) Genes Dev. 16, 1066-1076.
  5. Lin, C. et al. (2002) Cell 108, 837-847.
  6. Yanagawa, S. et al. (2002) EMBO J. 21, 1733-1742.
  7. Yost, C. et al. (1996) Genes Dev. 10, 1443-1454.
  8. Morin, P.J. (1997) Science 275, 1787-1790.
  9. Taurin, S. et al. (2006) J. Biol. Chem. 281, 9971-9976.
  10. Fang, D. et al. (2007) J. Biol. Chem. 282, 11221-11229.
  11. He, X.C. et al. (2007) Nat. Genet. 39, 189-198.
  12. Hino, S. et al. (2005) Mol. Cell Biol. 25, 9063-9072.

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