Cell Signaling Technology

Product Pathways - Development

TCF4 (L40C3) Mouse mAb #2953

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 58, 79 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TCF4 (L40C3) Mouse mAb detects endogenous levels of total TCF4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing aminals with a synthetic peptide corresponding to residues surrounding Glu81 of human TCF4.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TCF4 (L40C3) Mouse mAb.

Background

LEF1 and TCF are members of the high mobility group (HMG) DNA binding protein family of transcription factors that consists of the following: Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3, and TCF4 (1). LEF1 and TCF1 were originally identified as important factors regulating early lymphoid development (2) and act downstream in Wnt signaling. LEF1 and TCF bind to Wnt response elements to provide docking sites for β-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling (3). LEF1 and TCF are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers including colon cancer (4,5).

  1. Waterman, M.L. (2004) Cancer Metastasis Rev. 23, 41-52.
  2. Schilham, M.W. and Clevers, H. (1998) Semin. Immunol. 10, 127-132.
  3. Brantjes, H. et al. (2002) Biol. Chem. 383, 255-261.
  4. Reya, T. and Clevers, H. (2005) Nature 434, 843-850.
  5. Logan, C.Y. and Nusse, R. (2004) Annu. Rev. Cell Dev. Biol. 20, 781-810.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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