Product Pathways - Apoptosis
Smac/Diablo Mouse mAb #2954
|2954S||100 µl (10 western blots)||---||In Stock||---|
|2954||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Monkey||Endogenous||21||Mouse IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
Smac/Diablo Mouse mAb detects endogenous levels of processed and unprocessed human Smac/Diablo. The antibody reacts weakly with mouse and rat Smac/Diablo.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a full length peptide corresponding to human Smac/Diablo raised in E-coli.
Western blot analysis of extracts from 293, HeLa and Jurkat cells, using Smac/Diablo Mouse mAb.
Western blot analysis of extracts from HeLa cells, untreated (upper) or treated with Staurosporine #9953 (1 μM, 3 hr; lower), using Smac/Diablo Mouse mAb #2954. Cells were fractionated into whole cell lysate (WCL), cytoplasm (Cyto), membrane (Mem), and cytoskeletal/nucleus (Nuc) using Cell Fractionation Kit #9038. Membrane fraction includes mitochondria.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Smac/Diablo Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Smac/Diablo Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Smac/Diablo Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma, using Smac/Diablo Mouse mAb (left) or secondary alone (right).
Smac/Diablo is a 21 kDa mammalian mitochondrial protein that functions as a regulatory component during apoptosis (1,2). Upon mitochondrial stress, Smac/Diablo is released from mitochondria and competes with caspases for binding of IAPs (inhibitor of apoptosis proteins) (1,2). The interaction of Smac/Diablo with IAPs relieves the inhibitory effect of the IAPs on caspases (3,4). This interaction involves mainly the amino-terminal residues of Smac/Diablo with the BIR3 region of XIAP, supplemented with several other hydrophobic interactions between the helical structures of Smac/Diablo and other areas of BIR3 (5,6).
- Ogino, T. et al. (2009) Leuk Res 33, 151-8. Applications: Western Blotting.
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