Product Pathways - Apoptosis / Autophagy
Smac/Diablo Mouse mAb #2954
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC | H Mk | Endogenous | 21 | Mouse IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Smac/Diablo Mouse mAb detects endogenous levels of processed and unprocessed human Smac/Diablo. The antibody reacts weakly with mouse and rat Smac/Diablo.
Source / Purification
Monoclonal antibody is produced by immunizing mice with a full length peptide (KLH-coupled) corresponding to human Smac/Diablo raised in E-coli.
Western Blotting
Western blot analysis of extracts from 293, HeLa and Jurkat cells, using Smac/Diablo Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Smac/Diablo Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Smac/Diablo Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Smac/Diablo Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma, using Smac/Diablo Mouse mAb (left) or secondary alone (right).
IF-IC
Confocal immunofluorescent analysis of MCF-7 cells, using Smac/Diablo Mouse mAb (left, green) or with MitoTracker® Red CMXRos (right, red), which stains mitochondria, demonstrating colocalization. Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
Smac/Diablo is a 21 kDa mammalian mitochondrial protein that functions as a regulatory component during apoptosis (1,2). Upon mitochondrial stress, Smac/Diablo is released from mitochondria and competes with caspases for binding of IAPs (inhibitor of apoptosis proteins) (1,2). The interaction of Smac/Diablo with IAPs relieves the inhibitory effect of the IAPs on caspases (3,4). This interaction involves mainly the amino-terminal residues of Smac/Diablo with the BIR3 region of XIAP, supplemented with several other hydrophobic interactions between the helical structures of Smac/Diablo and other areas of BIR3 (5,6).
- Du, C. et al. (2000) Cell 102, 33-42.
- Verhagen, A.M. et al. (2000) Cell 102, 43-53.
- Srinivasula, S.M. et al. (2001) Nature 410, 112-116.
- Srinivasula, S.M. et al. (2000) J. Biol. Chem. 275, 36152-36157.
- Liu, Z. et al. (2000) Nature 408, 1004-1007.
- Wu, G. et al. (2000) Nature 408, 1008-1012.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
