Cell Signaling Technology

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GFP (4B10) Mouse mAb #2955

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P F All Transfected Only 27 Mouse IgG

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  F=Flow Cytometry
Reactivity Key: All=All species expected

Specificity / Sensitivity

GFP (4B10) Mouse mAb detects GFP-tagged proteins exogenously expressed in cells. Please note that the GFP tag adds approximately 27 kDa to the molecular weight of the fusion protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with full-length recombinant GFP.

Western Blotting

Western Blotting

Western blot analysis of extracts from HCC827 cells, untransfected or transfected with GFP, using GFP (4B10) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HCC827 cells, untransfected (left) or transfected with GFP (right) using GFP (4B10) Mouse mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HCC827 cells, untransfected (blue) or transfected with GFP (green), using GFP (4B10) Mouse mAb.


Background

The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineages and defines subcellular protein localizations (1).

  1. Miyawaki, A. et al. (2003) Curr Opin Chem Biol 7, 557-62.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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