Product Pathways - Complementary Reagents
GFP (4B10) Mouse mAb #2955
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P F | All | Transfected Only | 27 | Mouse IgG2a |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
F=Flow Cytometry
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
Protocols
- 2955:
- Flow, IHC / Paraffin, Western Blotting
Specificity / Sensitivity
GFP (4B10) Mouse mAb detects GFP-tagged proteins exogenously expressed in cells. Please note that the GFP tag adds approximately 27 kDa to the molecular weight of the fusion protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with full-length recombinant GFP.
Western Blotting
Western blot analysis of extracts from HCC827 cells, untransfected or transfected with GFP, using GFP (4B10) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded HCC827 cells, untransfected (left) or transfected with GFP (right) using GFP (4B10) Mouse mAb.
Flow Cytometry
Flow cytometric analysis of HCC827 cells, untransfected (blue) or transfected with GFP (green), using GFP (4B10) Mouse mAb.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineages and defines subcellular protein localizations (1).
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
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- 8112 SignalStain® Antibody Diluent
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
