Cell Signaling Technology

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GFP (D5.1) Rabbit mAb #2956

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P F All Transfected Only 27 Rabbit IgG

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  F=Flow Cytometry
Reactivity Key: All=All species expected

Specificity / Sensitivity

GFP (D5.1) Rabbit mAb detects GFP-tagged proteins exogenously expressed in cells.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide (KLH-coupled) corresponding to the amino terminus of GFP.

Western Blotting

Western Blotting

Western blot analysis of extracts from HCC827 cells, untransfected or GFP-transfected, using GFP (D5.1) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HCC827 cells, untransfected (left) or GFP-transfected (right), using GFP (D5.1) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HCC827 cells, untransfected (blue) or GFP-transfected (green), using GFP (D5.1) Rabbit mAb.


Background

The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage and defines subcellular protein localization (1).

  1. Miyawaki, A. et al. (2003) Curr Opin Chem Biol 7, 557-62.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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