Product Pathways - Complementary Reagents
GFP (D5.1) XP® Rabbit mAb #2956
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC F | All | Transfected Only | 27 | Rabbit IgG |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
Protocols
Specificity / Sensitivity
GFP (D5.1) XP® Rabbit mAb detects GFP-tagged proteins exogenously expressed in cells.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of GFP.
Western Blotting
Western blot analysis of extracts from HCC827 cells, untransfected or GFP-transfected, using GFP (D5.1) XP® Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded HCC827 cells, untransfected (left) or GFP-transfected (right), using GFP (D5.1) XP® Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of HCC827 cells, untransfected (blue) or GFP-transfected (green), using GFP (D5.1) XP® Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of COS-7 cells expressing a GFP-tagged fusion protein (green) using GFP (D5.1) XP® Rabbit mAb (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage and defines subcellular protein localization (1).
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2955 GFP (4B10) Mouse mAb
- 2372 β-Gal (14B7) Mouse mAb
- 2367 HA-Tag (6E2) Mouse mAb
- 2365 His-Tag Antibody
- 2276 Myc-Tag (9B11) Mouse mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
