Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Erlin-1 Antibody #2958

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 43 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Erlin-1 Antibody detects endogenous levels of total human erlin-1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly320 of human erlin-1. Antibodies are purified by peptide affinity chromatography.

Western blot analysis of extracts from human U937 and ovcar8 cell lines using Erlin-1 Antibody.

Background

Erlin-1 and erlin-2 (SPFH1 and SPFH2) are SPFH domain-containing proteins that belong to the prohibitin family (1,2). The N-termal domain of erlin proteins contains ER-targeting sequences responsible for their translocation to the endoplasmic reticulum (ER) (3). In the ER, erlin-1 and erlin-2 specifically associate with the detergent resistant lipid raft microdomain of the membrane (3). Erlin-1 may be involved in dentritic cell activation (4) and erlin-2 has been shown to regulate the ER-associated degradation (ERAD) pathway by interacting with endogenous substrates and resulting in their polyubiquitination and degradation (5).

  1. Browman, D.T. et al. (2007) Trends Cell Biol 17, 394-402.
  2. Morrow, I.C. and Parton, R.G. (2005) Traffic 6, 725-40.
  3. Browman, D.T. et al. (2006) J Cell Sci 119, 3149-60.
  4. Li, N. et al. (2000) Biochem Biophys Res Commun 279, 487-93.
  5. Pearce, M.M. et al. (2007) J Biol Chem 282, 20104-15.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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