Product Pathways - Translational Control
mTOR Antibody #2972
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 289 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
mTOR Antibody detects endogenous levels of total mTOR. It cross-reacts weakly with some other proteins based on Western analysis.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH coupled) corresponding to residues surrounding Ser2481 of human mTOR. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from 293 cells (starved for 16 hours), untreated or EGF-treated (100 ng/ml), using Phospho-mTOR (Ser2448) antibody #2971 (upper) or mTOR Antibody (lower).
Background
The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9).
- Sabers, C.J. et al. (1995) J. Biol. Chem. 270, 815-822.
- Brown, E.J. et al. (1994) Nature 369, 756-758.
- Sabatini, D.M. et al. (1994) Cell 78, 35-43.
- Gingras, A.C. et al. (2001) Genes Dev. 15, 807-826.
- Dennis, P.B. et al. (2001) Science 294, 1102-1105.
- Fang, Y. et al. (2001) Science 294, 1942-1945.
- Nave, B.T. et al. (1999) Biochem. J. 344 Pt 2, 427-431.
- Peterson, R.T. et al. (2000) J. Biol. Chem. 275, 7416-7423.
- Huang, S. and Houghton, P.J. (2003) Curr. Opin. Pharmacol. 3, 371-377.
Application References
- Suzuki, A. et al. (2004) ARK5 is a tumor invasion-associated factor downstream of Akt signaling. Mol. Cell. Biol. 24, 3526-3535. This article references the use of mTOR Antibody in the following applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2983 mTOR (7C10) Rabbit mAb
- 2971 Phospho-mTOR (Ser2448) Antibody
- 2974 Phospho-mTOR (Ser2481) Antibody
- 2976 Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific)
- 9904 Rapamycin (FRAP/mTOR Inhibitor)
- 9451 Phospho-4E-BP1 (Ser65) Antibody
- 2441 Phospho-eIF4G (Ser1108) Antibody
- 9205 Phospho-p70 S6 Kinase (Thr389) Antibody
- 2211 Phospho-S6 Ribosomal Protein (Ser235/236) Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
