Product Pathways - Translational Control
Phospho-mTOR (Ser2481) Antibody #2974
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W | H M R Mk | 289 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-mTOR (Ser2481) Antibody detects endogenous levels of mTOR only when phosphorylated at serine 2481.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH coupled) corresponding to residues surrounding Ser2481 of human mTOR. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from 293 cells (starved for 30 hours), untreated or treated with 20% FBS for 30 minutes, using Phospho-mTOR (Ser2481) Antibody (upper) or control mTOR Antibody #2972 (lower).
Background
The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9).
- Sabers, C.J. et al. (1995) J. Biol. Chem. 270, 815-822.
- Brown, E.J. et al. (1994) Nature 369, 756-758.
- Sabatini, D.M. et al. (1994) Cell 78, 35-43.
- Gingras, A.C. et al. (2001) Genes Dev. 15, 807-826.
- Dennis, P.B. et al. (2001) Science 294, 1102-1105.
- Fang, Y. et al. (2001) Science 294, 1942-1945.
- Nave, B.T. et al. (1999) Biochem. J. 344 Pt 2, 427-431.
- Peterson, R.T. et al. (2000) J. Biol. Chem. 275, 7416-7423.
- Huang, S. and Houghton, P.J. (2003) Curr. Opin. Pharmacol. 3, 371-377.
Application References
- Peterson, R. T. et al. (2000) FKBP12-rapamycin-associated protein (FRAP) autophosphorylates at serine 2481 under translationally repressive conditions. J. Biol. Chem. 275, 7416-7423. This article references the use of Phospho-mTOR (Ser2481) Antibody in the following applications: Western Blotting
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