Product Pathways - Translational Control
Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific) #2976
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| IHC-P IHC-F | H (M) (R) | Endogenous | 289 | Rabbit IgG |
Applications Key:
IHC-P=Immunohistochemistry (Paraffin)
IHC-F=Immunohistochemistry (Frozen)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-mTOR (Ser2448) (49F9) Rabbit mAb detects endogenous levels of mTOR protein only when phosphorylated at Ser2448.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser2448 of human mTOR.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma of the bladder using Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific) in the presence of control peptide (left) or Phospho-mTOR (Ser 2448) Blocking Peptide #1230 (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma untreated (left) or lambda phosphatase-treated (right), using Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific).
IHC-F (frozen)
Immunohistochemical analysis of frozen H1650 xenograft, showing cytoplasmic localization, using Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC specific).
Background
The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9).
- Sabers, C.J. et al. (1995) J. Biol. Chem. 270, 815-822.
- Brown, E.J. et al. (1994) Nature 369, 756-758.
- Sabatini, D.M. et al. (1994) Cell 78, 35-43.
- Gingras, A.C. et al. (2001) Genes Dev. 15, 807-826.
- Dennis, P.B. et al. (2001) Science 294, 1102-1105.
- Fang, Y. et al. (2001) Science 294, 1942-1945.
- Nave, B.T. et al. (1999) Biochem. J. 344 Pt 2, 427-431.
- Peterson, R.T. et al. (2000) J. Biol. Chem. 275, 7416-7423.
- Huang, S. and Houghton, P.J. (2003) Curr. Opin. Pharmacol. 3, 371-377.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2971 Phospho-mTOR (Ser2448) Antibody
- 2974 Phospho-mTOR (Ser2481) Antibody
- 2983 mTOR (7C10) Rabbit mAb
- 2972 mTOR Antibody
- 1230 Phospho-mTOR (Ser2448) Blocking Peptide
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
