Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

NOS (pan) Antibody #2977

Applications Reactivity Sensitivity MW (kDa) Source
W H M Endogenous 130, 140, 160 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

NOS (pan) Antibody detects endogenous levels of total eNOS, iNOS and nNOS protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Ser1000 of human iNOS. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Raw264.7 cells, untreated or LPS-treated (1 µg/ml for 24 h), mouse brain and EA.hy926 cells using NOS (pan) antibody.

Background

Nitric Oxide Synthase (NOS) catalyses the formation of nitric oxide (NO) and citruline from L-arginine, oxygen and cofactors. Three family members have been characterized: neuronal NOS (nNOS), which is found primarily in neuronal tissue; inducible NOS (iNOS), which is induced by interferon gamma and lipopolysaccharides in the kidney and cardiovascular system; and endothelial NOS (eNOS), which is expressed in blood vessels (1). NO is a messenger molecule with diverse functions throughout the body including the maintenance of vascular integrity, homeostasis, synaptic plasticity, long-term potentiation, learning, and memory (2,3).

  1. Tsutsui, M. (2004) J Atheroscler Thromb 11, 41-48.
  2. Son, H. et al. (1996) Cell 87, 1015-10123.
  3. Hawkins, R.D. (1996) Neuron 16, 465-467.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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