Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody #2981

Applications Reactivity Source
W IP E-P All Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  E-P=ELISA (Peptide)
Reactivity Key: All=All species expected

Specificity / Sensitivity

Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody detects endogenous levels of proteins containing the Arg-X-Tyr/Phe-X-pSer motif. This antibody does not cross-react with nonphosphorylated serine or phospho-threonine proteins with the same motif or other phospho-serine/threonine-containing proteins and peptides without this motif. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with Arg-X-Tyr/Phe-X-pSer-containing peptides (KLH-coupled). Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from A431 cells, untreated or calyculin A treated, using Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody.

Application References

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Companion Products

License/Use Restrictions: Use of CST Motif Antibodies within certain methods (e.g., U.S. Patent No.'s 7,198,896 & 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commerical licensing terms please contact CST Business Development at cbunker@cellsignal.com.

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