Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

iNOS Antibody (Mouse Specific) #2982

Applications Reactivity Sensitivity MW (kDa) Source
W IP M Endogenous 130 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

iNOS Antibody (Mouse Specific) detects endogenous levels of total iNOS protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide sourrounding Ala1130 of mouse iNOS. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Raw264.7 cells, untreated or LPS-treated (1 μg/ml for 6 h), using iNOS Antibody (Mouse Specific).

Western Blotting

Western Blotting

Western blot analysis of extracts from Raw264.7 cells, untreated or LPS-treated (1 µg/ml for 6 h), using iNOS Antibody (Mouse Specific).

Background

Nitric Oxide Synthase (NOS) catalyses the formation of nitric oxide (NO) and citruline from L-arginine, oxygen and cofactors. Three family members have been characterized: neuronal NOS (nNOS), which is found primarily in neuronal tissue; inducible NOS (iNOS), which is induced by interferon gamma and lipopolysaccharides in the kidney and cardiovascular system; and endothelial NOS (eNOS), which is expressed in blood vessels (1). NO is a messenger molecule with diverse functions throughout the body including the maintenance of vascular integrity, homeostasis, synaptic plasticity, long-term potentiation, learning, and memory (2,3).

Nitric oxide catalyzed by iNOS is involved in host defense against protozoa, bacteria, fungi and viruses. Unlike constitutively expressed eNOS and nNos, iNOS is not usually expressed in quiescent cells. iNOS is transcriptionally induced in response to bacterial endotoxins such as LPS and proinflammatory cytokines in macrophages and various other cell types. Transcription factors involved in iNOS transcription include NF-κB, AP-1 and STAT. Different signaling pathways either promote (Jak1/2, PKC, c-Raf, p38 MAP kinase and p44/42 MAP kinase) or inhibit (PI3 kinase) iNOS expression depending on stimulus and cell type (4).

  1. Tsutsui, M. (2004) J Atheroscler Thromb 11, 41-48.
  2. Son, H. et al. (1996) Cell 87, 1015-10123.
  3. Hawkins, R.D. (1996) Neuron 16, 465-467.
  4. Bogdan, C. (2001) Nat Immunol 2, 907-16.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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