Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Lymphocyte Signaling

Lck (D88) XP® Rabbit mAb #2984

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P H Endogenous 56 Rabbit IgG

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Lck (D88) XP® Rabbit mAb detects endogenous levels of total Lck protein. This antibody does not cross-react with other Src family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr50 of human Lck.

Western Blotting

Western Blotting

Western blot analysis of extracts from Ramos and Jurkat cells using LcK (D88) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Lck (D88) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lymphoma using Lck (D88) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Colo205 (positive) (left) and HeLa (negative) (right) cell pellets using Lck (D88) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human tonsil using Lck (D88) XP® Rabbit mAb.

Background

Lck belongs to the Src-like non-receptor tyrosine kinase family with the typical Src family kinase structure: a unique amino terminal domain (Src homology 4 domain, SH4) followed by an SH3 domain, an SH2 domain, a kinase domain (SH1), and a carboxy-terminal negative regulatory domain (1). Lck activity is controlled by the interactions of SH2 and SH3 domains as well as tyrosine phosphorylation status of the activation loop (2,3). Lck is recruited to the T cell receptor (TCR) complex upon stimulation and activates downstream tyrosine kinases to initiate T cell signaling (4). Lck is also found to be involved in the regulation of mitochondrial apoptosis pathways and may be responsible for some anticancer drug induced apoptosis (5,6).

  1. Palacios, E.H. and Weiss, A. (2004) Oncogene 23, 7990-8000.
  2. Mustelin, T. and Taskén, K. (2003) Biochem J 371, 15-27.
  3. Gervais, F.G. et al. (1993) Mol Cell Biol 13, 7112-21.
  4. Straus, D.B. and Weiss, A. (1992) Cell 70, 585-93.
  5. Belka, C. et al. (2003) Oncogene 22, 176-85.
  6. Gruber, C. et al. (2004) Biochem Pharmacol 67, 1859-72.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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