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Rabbit (DA1E) mAb IgG Isotype Control (Alexa Fluor® 647 Conjugate) #2985
| Applications | Reactivity | Isotype |
|---|---|---|
| F | Rabbit IgG |
Applications Key:
F=Flow Cytometry
Reactivity Key:
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Normal Rabbit (DA1E) mAb IgG is not expected to recognize any specific antigen. It functions as an isotype control for rabbit monoclonal antibodies.
Source / Purification
Flow Cytometry
Flow cytometric analysis of Jurkat cells, using Rabbit IgG Isotype Control (Alexa Fluor® 647 Conjugate) (red) compared to Phospho-Akt (Ser473) (D9E) Rabbit mAb (Alexa Fluor® 647 Conjugate) #4075, untreated (green) or LY294002- and wortmannin-treated (blue).
Flow Cytometry
Flow cytometric analysis of Jurkat cells, using Rabbit IgG Isotype Control (Alexa Fluor® 647 Conjugate) (red) compared to Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) Rabbit mAb (Alexa Fluor® 647 Conjugate) #4851, untreated (blue) or IFN-α-treated (green).
Description
Rabbit IgG was conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions, and tested in-house for direct flow cytometric analysis of human cells.
Background
Isotype control antibodies are used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type as the test antibody.
Application References
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Companion Products
- 3452 Rabbit IgG Isotype Control (Alexa Fluor® 647 Conjugate)
- 4075 Phospho-Akt (Ser473) (D9E) Rabbit mAb (Alexa Fluor® 647 Conjugate)
- 4851 Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) Rabbit mAb (Alexa Fluor® 647 Conjugate)
- 4284 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) Rabbit mAb (Alexa Fluor® 647 Conjugate)
- 2337 Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor® 647 Conjugate)
- 4324 Phospho-Stat3 (Tyr705) (D3A7) Rabbit mAb (Alexa Fluor® 647 Conjugate)
- 2975 Rabbit (DA1E) mAb IgG Isotype Control (Alexa Fluor® 488 Conjugate)
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
