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Rabbit (DA1E) mAb IgG XP™ Isotype Control (Alexa Fluor® 647 Conjugate) #2985
This XP™ monoclonal antibody was developed using our eXceptional Monoclonal Technology (XMT™).
| Applications | Reactivity | Isotype |
|---|---|---|
| F | Rabbit IgG |
Applications Key:
F=Flow Cytometry
Reactivity Key:
Species cross-reactivity is determined by Western blot.
Protocols
- 2985:
- Flow
Specificity / Sensitivity
Rabbit (DA1E) mAb IgG XP™ Isotype Control (Alexa Fluor® 647 Conjugate) is not expected to recognize any specific antigen. It functions as an isotype control for rabbit monoclonal antibodies.
Source / Purification
The purified antibody was conjugated to Alexa Fluor® 647 under optimal conditions with a F/P ratio of 2-6.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, using Rabbit (DA1E) mAb IgG XP™ Isotype Control (Alexa Fluor® 647 Conjugate) (red) compared to Phospho-Akt (Ser473) (D9E) XP™ Rabbit mAb (Alexa Fluor® 647 Conjugate) #4075, untreated (green) or LY294002- and wortmannin-treated (blue).
Flow Cytometry
Flow cytometric analysis of Jurkat cells, using Rabbit (DA1E) mAb IgG XP™ Isotype Control (Alexa Fluor® 647 Conjugate (red) compared to Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP™ Rabbit mAb (Alexa Fluor® 647 Conjugate) #4851, untreated (blue) or IFN-α-treated (green).
Description
This Cell Signaling Technology antibody was conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells.
Background
Isotype control antibodies are used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Application References
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Companion Products
- 3900 Rabbit (DA1E) mAb IgG XP™ Isotype Control
- 3452 Rabbit IgG Isotype Control (Alexa Fluor® 647 Conjugate)
- 4075 Phospho-Akt (Ser473) (D9E) XP™ Rabbit mAb (Alexa Fluor® 647 Conjugate)
- 4851 Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP™ Rabbit mAb (Alexa Fluor® 647 Conjugate)
- 4284 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP™ Rabbit mAb (Alexa Fluor® 647 Conjugate)
- 2337 Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor® 647 Conjugate)
- 4324 Phospho-Stat3 (Tyr705) (D3A7) XP™ Rabbit mAb (Alexa Fluor® 647 Conjugate)
- 2975 Rabbit (DA1E) mAb IgG XP™ Isotype Control (Alexa Fluor® 488 Conjugate)
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
