Product Pathways -
Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate) #2985
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-IC F | Endogenous | Rabbit IgG |
Applications Key:
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2985:
- Flow, Immunofluorescence
Specificity / Sensitivity
Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate) is not expected to recognize any specific antigen. It functions as an isotype control for rabbit monoclonal antibodies.
Source / Purification
The purified antibody was conjugated to Alexa Fluor® 647 under optimal conditions with a F/P ratio of 2-6.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, using Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate) (red) compared to Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #4075, untreated (green) or LY294002- and wortmannin-treated (blue).
Flow Cytometry
Flow cytometric analysis of Jurkat cells, using Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate (red) compared to Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #4851, untreated (blue) or IFN-α-treated (green).
IF-IC
Confocal immunofluorescent analysis of C2C12 cells using GAPDH (14C10) Rabbit mAb (Alexa Fluor® 647 Conjugate) #3907 (pseudocolor blue, left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate (pseudocolor blue, right). Red = Propidium Iodide (PI)/RNase Staining Solution #4087 (fluorescent DNA dye).
Description
This Cell Signaling Technology antibody was conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells.
Background
Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Application References
- Francisco, L.M. et al. (2009) J Exp Med 206, 3015-29. Applications: Flow Cytometry
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3900 Rabbit (DA1E) mAb IgG XP® Isotype Control
- 3452 Rabbit IgG Isotype Control (Alexa Fluor® 647 Conjugate)
- 4075 Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate)
- 4851 Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate)
- 4284 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate)
- 2337 Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor® 647 Conjugate)
- 4324 Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate)
- 2975 Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate)
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
