Cell Signaling Technology

Product Pathways - Neuroscience

CNPase Antibody #2986

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 47 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CNPase Antibody detects endogenous levels of total CNPase protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human CNPase. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brain using CNPase Antibody.

Background

CNPase (2', 3’-cyclic nucleotide 3'-phosphodiesterase) catalyzes the in vitro hydrolysis of 2’, 3’-cyclic nucleotides to produce 2’-nucleotides. The in vivo molecular function and native substrate of this nucleotide phosphodiesterase remains under investigation (1). High CNPase expression is seen in oligodendrocytes and Schwann cells as CNPase accounts for roughly 4% of the total myelin protein in the central nervous system (2). CNPase binds to tubulin heterodimers and plays a role in tubulin polymerization, and oligodendrocyte process outgrowth (3). Typical myelination is seen in CNPase knock-out mice, but they suffer severe neurodegeneration from axonal loss and oligodendrocytes display abnormal paranodal loop structure prior to axonal degeneration. Paranodal loops typically contact the axolemma in axon-glial signaling; neurodegeneration in CNPase knock-out mice is a secondary consequence of impaired cell-cell communication (4).

  1. Esposito, C. et al. (2008) Biochemistry 47, 308-19.
  2. Kozlov, G. et al. (2003) J Biol Chem 278, 46021-8.
  3. Lee, J. et al. (2005) J Cell Biol 170, 661-73.
  4. Lappe-Siefke, C. et al. (2003) Nat Genet 33, 366-74.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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