Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

RANTES (R40) Antibody #2987

Applications Reactivity Sensitivity MW (kDa) Source
W H (Mk) Endogenous 10 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

RANTES (R40) Antibody detects endogenous levels of total human RANTES protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg40 of human RANTES. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from differentiated THP-1 cells, untreated or treated overnight with LPS, using RANTES (R40) Antibody.

Western Blotting

Western Blotting

Western blot analysis of HeLa cells, mock transfected or transfected with human RANTES construct, using RANTES (R40) Antibody.

Background

RANTES/CCL5 (regulated upon activation, T cell expressed and secreted) is a member of the "C-C" or β family of chemokines that induce inflammation and are associated with a number of inflammatory disorders (1,2). RANTES is produced and secreted mainly by CD8+ T cells, macrophages, and platelets, as well as epithelial cells, fibroblasts and some solid tumors (2-7). RANTES acts as a chemoattractant and has other regulatory functions on a number of cell types including monocytes, memory T cells, NK cells, eosinophils, basophils, dendritic cells, and mast cells (3, 7-9). Signaling by RANTES is mediated by several G-protein coupled receptors (GPCRs), including CCR1, CCR3, CCR4 and CCR5.

  1. Schall, T.J. (1991) Cytokine 3, 165-83.
  2. Schall, T.J. et al. (1988) J Immunol 141, 1018-25.
  3. Kameyoshi, Y. et al. (1992) J Exp Med 176, 587-92.
  4. Rathanaswami, P. et al. (1993) J Biol Chem 268, 5834-9.
  5. Mattei, S. et al. (1994) Int J Cancer 56, 853-7.
  6. Stellato, C. et al. (1995) J Immunol 155, 410-8.
  7. Schall, T.J. et al. (1990) Nature 347, 669-71.
  8. Kuna, P. et al. (1992) J Immunol 149, 636-42.
  9. Mattoli, S. et al. (1995) Biochem Biophys Res Commun 209, 316-21.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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