Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

RANTES Antibody (Rodent Specific) #2989

Applications Reactivity Sensitivity MW (kDa) Source
W IP M (R) Transfected Only 10 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

RANTES Antibody (Rodent Specific) detects transfected levels of rodent RANTES.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxyl terminus of rodent RANTES.

Western Blotting

Western Blotting

Western blot analysis of HeLa cells, mock transfected or transfected with mouse RANTES, using RANTES Antibody (Rodent Specific).

Background

RANTES/CCL5 (regulated upon activation, T cell expressed and secreted) is a member of the "C-C" or β family of chemokines that induce inflammation and are associated with a number of inflammatory disorders (1,2). RANTES is produced and secreted mainly by CD8+ T cells, macrophages, and platelets, as well as epithelial cells, fibroblasts and some solid tumors (2-7). RANTES acts as a chemoattractant and has other regulatory functions on a number of cell types including monocytes, memory T cells, NK cells, eosinophils, basophils, dendritic cells, and mast cells (3, 7-9). Signaling by RANTES is mediated by several G-protein coupled receptors (GPCRs), including CCR1, CCR3, CCR4 and CCR5.

  1. Schall, T.J. (1991) Cytokine 3, 165-83.
  2. Schall, T.J. et al. (1988) J Immunol 141, 1018-25.
  3. Kameyoshi, Y. et al. (1992) J Exp Med 176, 587-92.
  4. Rathanaswami, P. et al. (1993) J Biol Chem 268, 5834-9.
  5. Mattei, S. et al. (1994) Int J Cancer 56, 853-7.
  6. Stellato, C. et al. (1995) J Immunol 155, 410-8.
  7. Schall, T.J. et al. (1990) Nature 347, 669-71.
  8. Kuna, P. et al. (1992) J Immunol 149, 636-42.
  9. Mattoli, S. et al. (1995) Biochem Biophys Res Commun 209, 316-21.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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