Product Pathways - Chromatin Regulation / Epigenetics
SUV39H1 Histone Methyltransferase Antibody #2991
PhosphoSitePlus® protein, site, and accession data: SUV39H1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M | Transfected Only | 48 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2991:
- Western Blotting
Specificity / Sensitivity
SUV39H1 Histone Methyltransferase Antibody detects transfected levels of total SUV39H1 histone methyltransferase. The antibody does not cross-react with other related proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human SUV39H1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of total extracts from COS cells, mock transfected or transfected with constructs expressing either human or mouse SUV39H1, using SUV39H1 Histone Methyltransferase Antibody.
Background
Human histone methyltransferase SUV39H1 is the homolog of the dominant Drosophila PEV modifier Su(var)3-9 and is composed of 412 amino acid residues (1). It combines two of the most evolutionarily conserved domains of the "chromatin regulators": the chromo and SET domains (2,3). The 60 amino acid chromo domain represents an ancient histone-like fold that directs heterochromatic localizations. It has been demonstrated that the 130 amino acid SET domain contains the methyltransferase catalytic motif, which cooperates with the adjacent cysteine-rich regions to confer histone methyltransferase activity (1). This enzyme selectively methylates histone H3 on Lys9, which generates a binding site for HP1 proteins, a family of heterochromatic adaptor molecules involved in both gene silencing and supra-nucleosomal chromatin structure (4,5). SUV39H1 histone methyltransferase plays an important role in modification of histone amino termini and regulation of gene expression.
- Rea, S. et al. (2000) Nature 406, 593-599.
- Koonin, E. V. et al. (1995) Nucleic Acids Res. 23, 4229-4232.
- Jenuwein, T. et al. (1998) Cell. Mol. Life Sci. 54, 80-93.
- Lachner, M. et al. (2001) Nature 410, 116-120.
- Bannister, A. J. et al. (2001) Nature 410, 120-123.
Application References
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
