Product Pathways - Complementary Reagents
HA-Tag (6E2) Mouse mAb (HRP Conjugate) #2999
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| W | All | Transfected Only | Mouse IgG1 |
Applications Key:
W=Western Blotting
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
Protocols
- 2999:
- Western Blotting
Specificity / Sensitivity
HA-Tag (6E2) Mouse mAb (HRP Conjugate) detects transfected HA-tagged proteins. The antibody recognizes the HA-tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA) . The antibody was conjugated to HRP under optimal conditions.
Western Blotting
Western blot analysis of extracts from COS cells, untransfected or transfected with HA-Akt (lane 2), using HA-Tag (6E2) Mouse mAb (HRP Conjugate).
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors (1).
- Field, J. et al. (1988) Mol. Cell. Biol. 8, 2159-2165.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
