Cell Signaling Technology

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HA-Tag (6E2) Mouse mAb (HRP Conjugate) #2999

Applications Reactivity Sensitivity Isotype
W H Mk Transfected Only Mouse IgG1

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

HA-Tag (6E2) Mouse mAb (HRP Conjugate) detects transfected HA-tagged proteins. The antibody recognizes the HA-tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA) . The antibody was conjugated to HRP under optimal conditions.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untransfected or transfected with HA-Akt (lane 2), using HA-Tag (6E2) Mouse mAb (HRP Conjugate).

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.

The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors (1).

  1. Field, J. et al. (1988) Mol. Cell. Biol. 8, 2159-2165.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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