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Phospho-p95/NBS1 (Ser343) Antibody #3001

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W E-P	H M Mi	Endogenous	95	Rabbit

Applications Key:  W=Western Blotting  E-P=ELISA (Peptide)
Reactivity Key:  H=Human  M=Mouse  Mi=Mink
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

3001:

ELISA Peptide, Western Blotting

Specificity / Sensitivity

Phospho-p95/NBS1 (Ser343) Antibody detects endogenous levels of p95/NBS1 only when phosphorylated at serine 343.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser343 of human p95/NBS1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The Nijmegen breakage syndrome (NBS) is characterized by defects in cell cycle checkpoints, growth retardation, an increased propensity for cancer and sensitivity to ionizing radiation (1). Repair of DNA double-strand breaks by radiation is dependent on a multifunctional complex containing Rad50, Mre11 and the NBS1 gene product p95/NBS1 (also called p95 or nibrin) (2). p95/NBS1 is a protein with a forkhead-associated domain and a carboxy-terminal repeat frequently found in cell cycle regulatory and DNA repair proteins (1,3). The overlap between clinical and cellular phenotypes in ataxia telangiectasia (AT) and NBS suggests that AT-mutated (ATM) and p95/NBS1 function in the same biochemical pathway. ATM interacts with and phosphorylates p95/NBS1 at Ser343 after exposure to ionizing radiation (4-7).

1. Varon, R. et al. (1998) Cell 93, 467-476.
2. Carney, J. P. et al. (1998) Cell 93, 477-486.
3. Durocher, D. et al. (1999) Mol. Cell 4, 387-394.
4. Gatei, M. et al. (2000) Nat. Genet. 25, 115-119.
5. Lim, D. S. et al. (2000) Nature 404, 613-617.
6. Wu, X. et al. (2000) Nature 405, 477-482.
7. Zhao, S. et al. (2000) Nature 405, 473-477.

Application References

• Lukas, C. et al. (2003) Distinct spatiotemporal dynamics of mammalian checkpoint regulators induced by DNA damage. Nat. Cell Biol. 5, 255-260. Applications: IC-IF Western Blotting

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Companion Products

• 2341 Phospho-Chk1 (Ser345) Antibody
• 2661 Phospho-Chk2 (Thr68) Antibody
• 9284 Phospho-p53 (Ser15) Antibody
• 9286 Phospho-p53 (Ser15) (16G8) Mouse mAb
• 9287 Phospho-p53 (Ser20) Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

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For Research Use Only. Not For Use In Diagnostic Procedures.