Product Pathways - DNA Damage
Phospho-p95/NBS1 (Ser343) Antibody #3001
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W E-P | H M Mi | Endogenous | 95 | Rabbit |
Applications Key:
W=Western Blotting
E-P=ELISA (Peptide)
Reactivity Key:
H=Human
M=Mouse
Mi=Mink
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-p95/NBS1 (Ser343) Antibody detects endogenous levels of p95/NBS1 only when phosphorylated at serine 343.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser343 of human p95/NBS1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from MvILu cells treated with UV or hydroxyurea (HU) for the indicated times, using Phospho-p95/NBS1 (Ser343) Antibody.
Background
The Nijmegen breakage syndrome (NBS) is characterized by defects in cell cycle checkpoints, growth retardation, an increased propensity for cancer and sensitivity to ionizing radiation (1). Repair of DNA double-strand breaks by radiation is dependent on a multifunctional complex containing Rad50, Mre11 and the NBS1 gene product p95/NBS1 (also called p95 or nibrin) (2). p95/NBS1 is a protein with a forkhead-associated domain and a carboxy-terminal repeat frequently found in cell cycle regulatory and DNA repair proteins (1,3). The overlap between clinical and cellular phenotypes in ataxia telangiectasia (AT) and NBS suggests that AT-mutated (ATM) and p95/NBS1 function in the same biochemical pathway. ATM interacts with and phosphorylates p95/NBS1 at Ser343 after exposure to ionizing radiation (4-7).
- Varon, R. et al. (1998) Cell 93, 467-476.
- Carney, J. P. et al. (1998) Cell 93, 477-486.
- Durocher, D. et al. (1999) Mol. Cell 4, 387-394.
- Gatei, M. et al. (2000) Nat. Genet. 25, 115-119.
- Lim, D. S. et al. (2000) Nature 404, 613-617.
- Wu, X. et al. (2000) Nature 405, 477-482.
- Zhao, S. et al. (2000) Nature 405, 473-477.
Application References
- Lukas, C. et al. (2003) Distinct spatiotemporal dynamics of mammalian checkpoint regulators induced by DNA damage. Nat. Cell Biol. 5, 255-260. This article references the use of Phospho-p95/NBS1 (Ser343) Antibody in the following applications: IC-IF Western Blotting
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
