Cell Signaling Technology

Product Pathways - DNA Damage

p95/NBS1 Antibody #3002

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Mi Endogenous 95 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Mi=Mink
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

p95/NBS1 Antibody detects endogenous levels of total p95/NBS1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human p95/NBS1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS, PC12, NIH3T3, HeLa and MvILu cells, using p95/NBS1 Antibody.

Background

The Nijmegen breakage syndrome (NBS) is characterized by defects in cell cycle checkpoints, growth retardation, an increased propensity for cancer and sensitivity to ionizing radiation (1). Repair of DNA double-strand breaks by radiation is dependent on a multifunctional complex containing Rad50, Mre11 and the NBS1 gene product p95/NBS1 (also called p95 or nibrin) (2). p95/NBS1 is a protein with a forkhead-associated domain and a carboxy-terminal repeat frequently found in cell cycle regulatory and DNA repair proteins (1,3). The overlap between clinical and cellular phenotypes in ataxia telangiectasia (AT) and NBS suggests that AT-mutated (ATM) and p95/NBS1 function in the same biochemical pathway. ATM interacts with and phosphorylates p95/NBS1 at Ser343 after exposure to ionizing radiation (4-7).

  1. Varon, R. et al. (1998) Cell 93, 467-476.
  2. Carney, J. P. et al. (1998) Cell 93, 477-486.
  3. Durocher, D. et al. (1999) Mol. Cell 4, 387-394.
  4. Gatei, M. et al. (2000) Nat. Genet. 25, 115-119.
  5. Lim, D. S. et al. (2000) Nature 404, 613-617.
  6. Wu, X. et al. (2000) Nature 405, 477-482.
  7. Zhao, S. et al. (2000) Nature 405, 473-477.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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