Product Pathways - DNA Damage
p95/NBS1 Antibody #3002
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk Mi | Endogenous | 95 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Mi=Mink
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
p95/NBS1 Antibody detects endogenous levels of total p95/NBS1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to carboxy-terminal residues of human p95/NBS1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from COS, PC12, NIH3T3, HeLa and MvILu cells, using p95/NBS1 Antibody.
Background
The Nijmegen breakage syndrome (NBS) is characterized by defects in cell cycle checkpoints, growth retardation, an increased propensity for cancer and sensitivity to ionizing radiation (1). Repair of DNA double-strand breaks by radiation is dependent on a multifunctional complex containing Rad50, Mre11 and the NBS1 gene product p95/NBS1 (also called p95 or nibrin) (2). p95/NBS1 is a protein with a forkhead-associated domain and a carboxy-terminal repeat frequently found in cell cycle regulatory and DNA repair proteins (1,3). The overlap between clinical and cellular phenotypes in ataxia telangiectasia (AT) and NBS suggests that AT-mutated (ATM) and p95/NBS1 function in the same biochemical pathway. ATM interacts with and phosphorylates p95/NBS1 at Ser343 after exposure to ionizing radiation (4-7).
- Varon, R. et al. (1998) Cell 93, 467-476.
- Carney, J. P. et al. (1998) Cell 93, 477-486.
- Durocher, D. et al. (1999) Mol. Cell 4, 387-394.
- Gatei, M. et al. (2000) Nat. Genet. 25, 115-119.
- Lim, D. S. et al. (2000) Nature 404, 613-617.
- Wu, X. et al. (2000) Nature 405, 477-482.
- Zhao, S. et al. (2000) Nature 405, 473-477.
Application References
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Companion Products
- 3001 Phospho-p95/NBS1 (Ser343) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
