Product Pathways - MAPK Signaling
Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb #3007
PhosphoSitePlus® protein, site, and accession data: MAPKAPK2
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC F | H M R Mk | Endogenous | 49 | Rabbit IgG |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb detects endogenous levels of MAPKAPK-2 protein only when phosphorylated at threonine 334.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr334 of human MAPKAPK-2.
Western Blotting
Western blot analysis of extracts from untreated or UV+TPA-treated HeLa and COS cells, using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (upper), or MAPKAPK-2 Antibody #3042 (lower).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing nuclear localization, using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or λ-phosphatase-treated (right), using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb in the presence of control peptide (left) or Phospho-MAPKAPK-2 (Thr334) Blocking Peptide #1041 (right).
Flow Cytometry
Flow cytometric analysis of HeLa cells, untreated (blue) or UV-treated (green), using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of HeLa cells, untreated (left), UV-treated (center) or UV and lambda phosphatase-treated (right), using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Background
In response to cytokines, stress, and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay (2). Phosphorylation at Thr222, Ser272, and Thr334 appears to be essential for the activity of MAPKAPK-2 (2). Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2).
Application References
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Companion Products
- 3042 MAPKAPK-2 Antibody
- 3041 Phospho-MAPKAPK-2 (Thr334) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
For Research Use Only. Not For Use In Diagnostic Procedures.
