Cell Signaling Technology

Product Pathways - Apoptosis

DAPK1 Antibody #3008

Applications Reactivity Sensitivity MW (kDa) Source
W H M (R) (Mk) Endogenous 160 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

DAPK1 Antibody detects endogenous levels of total DAPK1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly903 of human DAPK1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using DAPK1 Antibody.

Background

Death-associated protein kinase (DAPK1) is a Ca2+/calmodulin-regulated serine/threonine kinase that participates in a wide range of apoptotic signals including interferon-γ, tumor necrosis factor α, Fas, activated c-Myc, and detachment from the extracellular matrix. In addition to the kinase domain and calmodulin regulatory segment, DAPK1 also has eight ankyrin repeats, a cytoskeleton binding region, and a conserved death domain (1-3). Deletion of the calmodulin-regulatory domain generates a constitutively active mutant kinase. Ectopic expression of wild-type DAPK1 induced cell death in HeLa cells. Conversely, expression of a catalytically inactive mutant protected cells from interferon-γ-induced cell death (4). The catalytic domain of DAPK1 has very high sequence similarity to vertebrate myosin light chain kinase (MLCK) and a RXX(S/T)X motif derived from myosin light chain protein was shown to be phosphorylated in vitro by DAPK1 (5).

Epigenetic silencing of DAPK1 by promoter methylation has been observed in cases of chronic lymphocytic leukemia (6,7).

  1. Kimchi, A. (1999) Ann Rheum Dis. 58, I14-I19.
  2. Cohen, O. et al. (1999) J Cell Biol 146, 141-148.
  3. Deiss, L. P. et al. (1995) Genes Dev 9, 15-30.
  4. Cohen, O. et al. (1997) EMBO J 16, 998-1008.
  5. Velentza, A. V. et al. (2001) J Biol Chem 276, 38956-38965.
  6. Raval, A. et al. (2007) Cell 129, 879-890.
  7. Katzenellenbogen, R.A. et al. (1999) Blood 93, 4347-4353.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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