Product Pathways - NF-kappaB Signaling
TBK1/NAK Antibody #3013
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 84 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
TBK1 Antibody detects endogenous levels of total TBK1/NAK protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues near the carboxy-terminus of TBK1/NAK. Antibodies were purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa (human), BaF3 (mouse) and KNRK (rat) cell lines, using TBK1/NAK Antibody.
Background
TBK1 (TANK-binding kinase 1)/NAK (NF-kappaB activating kinase) is an IkappaB kinase (IKK)-activating kinase and can activate IKK through direct phosphorylation (1). TBK1 was identified through association with the TRAF binding protein, TANK and found to function upstream of NIK and IKK in the activation of NF-kappaB (2). TBK1/NAK induces IkappaB degradation and NF-kappaB activity through IKKbeta. NAK may mediate IKK and NF-kappaB activation in response to growth factors that stimulate PKCepsilon activity (1). TBK1 plays a pivotal role in the activation of IRF3 in the innate immune response (3).
- Tojima, Y. et al. (2000) Nature 404, 778-782.
- Pomerantz, J.L. and Baltimore, D. (1999) EMBO J 18, 6694-704.
- Fitzgerald, K.A. et al. (2003) Nat Immunol 4, 491-6.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
