Product Pathways - Metabolism
Insulin (C27C9) Rabbit mAb #3014
PhosphoSitePlus® protein, site, and accession data: insulin
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| IHC-P IF-F IF-IC F | H M R | Endogenous | 6 | Rabbit IgG |
Applications Key:
IHC-P=Immunohistochemistry (Paraffin)
IF-F=Immunofluorescence (Frozen)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3014:
- Flow, IHC / Paraffin, Immunofluorescence
Specificity / Sensitivity
Insulin (C27C9) Rabbit mAb detects endogenous levels of total insulin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human insulin.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human pancreas, showing the staining of β cells, using Insulin (C27C9) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical anaylsis of paraffin-embedded mouse pancreas, showing staining of β cells, using Insulin (C27C9) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded rat pancreas, showing staining of β cells, using Insulin (C27C9) Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of HeLa cells (blue) and β-TC-6 cells (green) using Insulin (C27C9) Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of β-TC-6 (left) and C2C12 cells (right) using Insulin (C27C9) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
IF-F
Confocal immunofluorescent analysis of rat pancreas using Insulin (C27C9) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
The maintenance of glucose homeostasis is an essential physiological process that is regulated by hormones. An elevation in blood glucose levels during feeding stimulates insulin release from pancreatic β cells through a glucose sensing pathway (1). Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Insulin stimulates glucose uptake from blood into skeletal muscle and adipose tissue. Insulin deficiency leads to type 1 diabetes mellitus (2).
- Straub, S.G. and Sharp, G.W. (2002) Diabetes Metab. Res. Rev. 18, 451-463.
- Concannon, P. et al. (1998) Nat. Genet. 19, 292-296.
Application References
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Companion Products
- 4590 Insulin Antibody
- 4593 C-Peptide Antibody
- 2760 Glucagon Antibody
- 8112 SignalStain® Antibody Diluent
For Research Use Only. Not For Use In Diagnostic Procedures.
