Product Pathways - Tyrosine Kinase / Adaptors
Met Signaling Antibody Sampler Kit #3019
|Kit Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|Phospho-Gab1 (Tyr307) Antibody #3234||40 µl||W||H M||115||Rabbit|
|Gab1 Antibody #3232||40 µl||W IP||H M R Mk||110||Rabbit|
|Phospho-Met (Tyr1003) (13D11) Rabbit mAb #3135||40 µl||W||H M R||145||Rabbit IgG|
|Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb #3077||40 µl||W IP IHC-P IHC-F IF-IC F||H M R||145||Rabbit|
|Phospho-Met (Tyr1349) (130H2) Rabbit mAb #3133||40 µl||W||H M R||145||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
|Met (D1C2) XP® Rabbit mAb #8198||40 µl||W IP IHC-P IHC-F IF-IC F||H||140, 170||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Each antibody in the Met Signaling Antibody Sampler Kit recognizes endogenous levels of its specific target and does not cross-react with other family members unless otherwise indicated. Phospho-Met (Tyr1234/1235) (D26) Rabbit mAb may cross-react with overexpressed tyrosine phosphorylated Src proteins in Western blot. Phospho-Met (Tyr1349) (130H2) Rabbit mAb may cross-react with other activated protein tyrosine kinases. Phospho-Met (Tyr1003) (13D11) Rabbit mAb may cross-react with other activated protein tyrosine kinases. Phospho-Gab1 (Tyr307) Antibody cross-reacts with phosphorylated Gab2 and potentially with phosphorylated Gab3.
Western blot analysis of cell extracts from HeLa cells, untreated or stimulated with HGF, using Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb #3077 (upper) and Met (25H2) Mouse mAb #3127 (lower).
Western blot analysis of cell lysates of H4IIE cells, untreated or treated with HGF, using Phospho-Met (Tyr1349) (130H2) Rabbit mAb #3133 (upper), or Met (25H2) Mouse mAb #3127 (lower).
Western blot analysis of extracts from A431 cells, untreated or HGF-treated, using Phospho-Met (Tyr1003) (13D11) Rabbit mAb #3135 (upper and middle) and Met (25H2) Mouse mAb #3127 (lower). The middle blot was treated with CIP phosphatase before antibody probing.
Western blot analysis of extracts from various cell lines using Gab1 Antibody #3232.
Western blot analysis of extracts from HEK293 cells, untreated or treated with In1B for indicated times, using Phospho-Gab1 (Tyr307) Antibody #3234 (upper) or Gab1 Antibody #3232 (lower).
Western blot analysis of extracts from HT-29 (Met+), SK-BR-3 (Met-), and T-47D (Met-) cells using Met (D1C2) XP® Rabbit mAb #8198 (upper) or β-Actin Antibody #4967 (lower).
The Met Signaling Antibody Sampler Kit provides an economical means to investigate Met signaling. The kit contains primary and secondary antibodies to perform four western blots with each antibody.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides or peptides corresponding to residues surrounding: Tyr1003, Tyr1234/1235, Tyr1349, or near the carboxy terminus of human Met protein. Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide or peptide corresponding to residues surrounding Tyr472 and Tyr307 of human Gab1. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor), is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. Addition of a phosphate at cytoplasmic Tyr1003 is essential for ubiquitination and Met protein degradation (4). Phosphorylation of Tyr1234/1235 in the Met kinase domain is critical to kinase activation. Phosphorylation of Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon and breast cancers. Thus, Met is an attractive cancer therapeutic and diagnostic target (6).
- Weidner, K.M. et al. (1993) Mol Immunol 30, 1003-11.
- Park, M. et al. (1986) Cell 45, 895-904.
- Bardelli, A. et al. (1997) Oncogene 15, 3103-11.
- Taher, T.E. et al. (2002) J Immunol 169, 3793-800.
- Schaeper, U. et al. (2000) J Cell Biol 149, 1419-32.
- Traxler, P. et al. (2001) Med Res Rev 21, 499-512.
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- 3148 Met (L41G3) Mouse mAb
- 3126 Phospho-Met (Tyr1234/1235) Antibody
- 3121 Phospho-Met (Tyr1349) Antibody
- 4560 Met Antibody
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
Selected rabbit monoclonal antibodies are produced under license (granting certain rights including those under U. S. Patent No. 5,675,063 and/or U.S.S.N. 11/476,277) from Epitomics, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.