Product Pathways - NF-kappaB Signaling

Phospho-NF-κB p65 (Ser536) Antibody #3031

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Applications	Reactivity	Sensitivity	MW (kDa)	Source
W	H M R Mk (Dg)	Endogenous	65	Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Dg=Dog
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-NF-kappaB p65 (Ser536) Antibody detects NF-kappaB p65 only when phosphorylated at serine 536. The antibody does not cross-react with the p50 subunit or other related proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser536 of human NF-kappaB p65. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Transcription factors of the nuclear factor kappaB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50) and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through an ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKK-α (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).

1. Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
2. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
3. Haskill, S. et al. (1991) Cell 65, 1281-9.
4. Thompson, J.E. et al. (1995) Cell 80, 573-82.
5. Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63.
8. Chen, Z.J. et al. (1996) Cell 84, 853-62.
9. Senftleben, U. et al. (2001) Science 293, 1495-9.
10. Coope, H.J. et al. (2002) EMBO J 21, 5375-85.
11. Xiao, G. et al. (2001) Mol Cell 7, 401-9.

Application References

• Haller, D. et al. (2002) J Biol Chem 277, 38168-78. This article references the use of Phospho-NF-κB p65 (Ser536) Antibody in the following applications: Western Blotting
• Strassheim, D. et al. (2004) J Immunol 172, 5727-33. This article references the use of Phospho-NF-κB p65 (Ser536) Antibody in the following applications: Western Blotting
• Haller, D. et al. (2003) J Biol Chem 278, 23851-60. This article references the use of Phospho-NF-κB p65 (Ser536) Antibody in the following applications: IHC-P (paraffin) Western Blotting
• Ruiz, P.A. et al. (2004) J Biol Chem 279, 36103-11. This article references the use of Phospho-NF-κB p65 (Ser536) Antibody in the following applications: Western Blotting
• Muller, I. et al. (2003) Cancer Res 63, 1772-5. This article references the use of Phospho-NF-κB p65 (Ser536) Antibody in the following applications: Western Blotting
• Anwar, K.N. et al. (2004) J Immunol 173, 6965-72. This article references the use of Phospho-NF-κB p65 (Ser536) Antibody in the following applications: Western Blotting

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