Product Pathways - NF-kappaB Signaling
Phospho-NF-κB p65 (Ser536) (7F1) Mouse mAb #3036
| Applications | Reactivity | Sensitivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|---|
| W | H M R Mk Mi (Dg) | Endogenous | 65 | Mouse | IgG2b |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Mi=Mink
Dg=Dog
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-NF-kappaB p65 (Ser536) (7F1) Mouse mAb detects NF-kappaB p65 only when phosphorylated at serine 536. It does not cross-react with the p50 subunit or other related proteins.
Source / Purification
Monoclonal antibody is produced by immunizing mice with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser536 of human NF-kappaB p65.
Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or TNF-alpha-treated (#2169, 20ng/ml), using Phospho-NF-kappaB p65 (Ser536) (7F1) Mouse mAb (upper) or NF-kappaB p65 Antibody #3034 (lower).
Background
Transcription factors of the nuclear factor kappaB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50) and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through an ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKK-α (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).
- Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
- Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
- Haskill, S. et al. (1991) Cell 65, 1281-9.
- Thompson, J.E. et al. (1995) Cell 80, 573-82.
- Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26.
- Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
- Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63.
- Chen, Z.J. et al. (1996) Cell 84, 853-62.
- Senftleben, U. et al. (2001) Science 293, 1495-9.
- Coope, H.J. et al. (2002) EMBO J 21, 5375-85.
- Xiao, G. et al. (2001) Mol Cell 7, 401-9.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 4764 NF-κB p65 (C22B4) Rabbit mAb
- 3034 NF-κB p65 Antibody
- 3033 Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb
- 3037 Phospho-NF-κB p65 (Ser276) Antibody
- 3039 Phospho-NF-κB p65 (Ser468) Antibody
- 4814 IκB-α (L35A5) Mouse mAb (Amino-terminal Antigen)
- 2859 Phospho-IκBα (Ser32) (14D4) Rabbit mAb
- 9243 NF-κB Control Cell Extracts
- 9936 NF-κB Pathway Sampler Kit
- 2169 Tumor Necrosis Factor-α
- 7072 Phototope®-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
